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AB259386

Recombinant mouse Interferon alpha 1 protein (Active)

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Recombinant mouse Interferon alpha 1 protein (Active) is a Mouse Full Length protein, in the 24 to 189 aa range, expressed in HEK 293 cells, with >95%, < 0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

대체 명칭 보기

Interferon alpha-1, IFN-alpha-1, Ifna1

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Mass Spectrometry - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)

M-Arg + 0.3 Da (loss of C-terminal lysine during analysis). Calculated MW is 19175.70 Da.

Functional Studies - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)

Cytopathic Effect Inhibition Assay of L929 cells against Encephalomyocarditis virus.

ED50 < 7 x 10-3 ng/mL, corresponding to a specific activity of 1.43 x 108 units/mg.

SDS-PAGE - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)

SDS-PAGE analysis of ab259386.

HPLC - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)
  • HPLC

Supplier Data

HPLC - Recombinant mouse Interferon alpha 1 protein (Active) (AB259386)

Purity by HPLC ≥95%

주요 정보

Purity

>95% SDS-PAGE

Purity by HPLC = 95%

Endotoxin 레벨

< 0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

Cell Culture, SDS-PAGE, HPLC, FuncS, Mass Spec

applications

Biologically active

Yes

Biological activity

Cytopathic Effect Inhibition Assay of L929 cells against Encephalomyocarditis virus.

ED50 < 7 x 10-3 ng/mL, corresponding to a specific activity of 1.43 x 108 units/mg.

Accession

Animal free

Yes

Carrier free

No

Species

Mouse

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 6 - 8 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

  • 0
  • 0
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Recombinant Human Interferon alpha 2b protein (Active)

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Functional Studies - Recombinant Human Interferon alpha 2b protein (Active) (AB309956)

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AB259405

Recombinant mouse IL-3 protein (Active)

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Mass Spectrometry - Recombinant mouse IL-3 protein (Active) (AB259405)

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Proteins & Peptides

AB316396

Recombinant Human Interferon beta Protein Standard (10x His tag)

Sandwich ELISA - Recombinant Human Interferon beta Protein Standard (10x His tag) (AB316396)

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Proteins & Peptides

AB259377

Recombinant Human Interferon gamma protein (Active)

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Functional Studies - Recombinant Human Interferon gamma protein (Active) (AB259377)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.
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서열 정보

[{"linker":null,"sequence":"CDLPQTHNLRNKRALTLLVQMRRLSPLSCLKDRKDFGFPQEKVDAQQIKKAQAIPVLSELTQQILNIFTSKDSSAAWNTTLLDSFCNDLHQQLNDLQGCLMQQVGVQEFPLTQEDALLAVRKYFHRITVYLREKKHSPCAWEVVRAEVWRALSSSANVLGRLREEK","proteinLength":"Full Length","predictedMolecularWeight":"19.18 kDa","actualMolecularWeight":null,"aminoAcidEnd":189,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P01572","tags":[]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 1 (IFN-α1) is a type I interferon with significant antiviral and immunomodulatory activities. Alternate names include interferon alpha and IFNA1. This protein weighs approximately 19 kDa and expresses predominantly in leukocytes. It interacts with specific cell surface receptors to initiate various protective mechanisms in the cell. Researchers often study IFN-alpha-1 protein using techniques like interferon alpha ELISA and IFN-α ELISA for its effective quantification in experimental analyses.
Biological function summary

Interferon alpha 1 functions as an important player in the immune response against viral infections. It belongs to a family of cytokines that signal through the JAK-STAT pathway to induce the expression of interferon-stimulated genes (ISGs). IFN-α1 operates independently and does not form large complexes with other proteins ensuring a focused response to pathogens. This protein's role is critical for the initiation of immune responses and limiting the spread of viruses within the host organism.

Pathways

Interferon alpha 1 activates intracellular signaling cascades mainly the JAK-STAT pathway and NF-κB pathway. These pathways have important roles in cellular defense mechanisms. In the JAK-STAT pathway IFN-α1 interacts closely with other interferons and cytokines to enhance antiviral states. Through these pathways it shares functional relationships with proteins such as STAT1 and IRF7 which are critical for mounting effective antiviral defenses.

Interferon alpha 1 relates closely to conditions like hepatitis C and multiple sclerosis. It has therapeutic applications in these diseases due to its immunoregulatory effects. IFN-α1 interacts with various immune system components including major histocompatibility complex (MHC) proteins to modulate immune responses. Abnormalities in IFN-α1 signaling or expression can exacerbate inflammatory and autoimmune disorders linking it to both protective and pathological roles in human health.
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일반 정보

기능

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.

서열 유사성

Belongs to the type-I (or alpha/beta) interferon family.

Post-translational modifications

Glycosylated.

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제품 프로토콜

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타겟 정보

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information Ifna1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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