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AB196418

Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus)

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Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) is a Mouse Fragment protein, in the 30 to 397 aa range, expressed in Baculovirus infected Sf9 cells, with >38%, suitable for SDS-PAGE, FuncS.

대체 명칭 보기

Cot, Tpl2, Map3k8, Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2

2 이미지
Functional Studies - Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) (AB196418)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) (AB196418)

Activity assay of ab196418.

SDS-PAGE - Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) (AB196418)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) (AB196418)

10% SDS-PAGE analysis of 2.5 μg ab196418 with Coomassie staining.

주요 정보

Purity

>38% SDS-PAGE

Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

His tag N-Terminus GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific Activity: 8.5 pmole/min/mg.

Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA) containing 5 μg inactive MEK1, 20 μM ATP, and ab196418 for 60 minutes at 30°C. The amount of ATP transferred was measured.

Accession

Animal free

No

Carrier free

No

Species

Mouse

보관 버퍼

pH: 8 Preservative: 0.91% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.15% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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보완 제품

Primary Antibodies

AB308011

Anti-MAP3K8/COT antibody [RP23040075]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)

  • 0
  • 0
Primary Antibodies

AB192667

Anti-MAP3K8/COT (phospho S400) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K8/COT (phospho S400) antibody (AB192667)

  • 0
  • 0
Primary Antibodies

AB263947

Anti-STAT6 (phospho Y641) antibody [EPR22599-78]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (AB263947)

  • 5
  • 1
Proteins & Peptides

AB287921

Recombinant human Integrin alpha V + beta 3 protein

SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (AB287921)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product was previously labelled as MAP3K8
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서열 정보

[{"linker":null,"sequence":"MENLYASEEPGVYEPSLMTMYPDSNQNEERSESLLRSGQEVPWLSSVRYGTVEDLLAFANHVSNMTKHFYGRRPQECGILLNMVISPQNGRYQIDSDVLLVPWKLTYRNIGSGFVPRGAFGKVYLAQDMKTKKRMACKLIPIDQFKPSDVEIQACFRHENIAELYGAVLWGDTVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHILKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVKMTEDVYLPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIAGDCSPGMRELIEAALERNPNHRPKAADLLKHEALNPPREDQPR","proteinLength":"Fragment","predictedMolecularWeight":"70 kDa","actualMolecularWeight":null,"aminoAcidEnd":397,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q07174","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
보관 정보
Avoid freeze / thaw cycle
True
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일반 정보

기능

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle.

서열 유사성

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Post-translational modifications

Autophosphorylated.

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제품 프로토콜

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타겟 정보

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle.
See full target information Map3k8
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Product promise

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