JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB277014

Recombinant Mouse SMPDL3A protein (His tag)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Recombinant Mouse SMPDL3A protein (His tag) is a Mouse Full Length protein, in the 1 to 445 aa range, expressed in HEK 293 cells, with >95%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

대체 명칭 보기

Asml3a, Smpdl3a, Cyclic GMP-AMP phosphodiesterase SMPDL3A, Acid sphingomyelinase-like phosphodiesterase 3a, ASM-like phosphodiesterase 3a

1 이미지
SDS-PAGE - Recombinant Mouse SMPDL3A protein (His tag) (AB277014)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse SMPDL3A protein (His tag) (AB277014)

SDS-PAGE analysis of ab277014

주요 정보

Purity

>95% SDS-PAGE

Endotoxin 레벨

< 1 EU/µg

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Mouse

보관 버퍼

pH: 7.4 Constituents: PBS

storage-buffer

style
left-column

보완 제품

Proteins & Peptides

AB245929

Recombinant Human Rab5 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Rab5 protein (His tag N-Terminus) (AB245929)

  • 5
  • 1
Proteins & Peptides

AB152060

Recombinant Human CD98 protein

  • 0
  • 0
Proteins & Peptides

AB124588

Recombinant Human Amyloid Precursor Protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Amyloid Precursor Protein (His tag N-Terminus) (AB124588)

  • 0
  • 0
Proteins & Peptides

AB315990

Recombinant Human CD74 Protein Standard (His tag)

Sandwich ELISA - Recombinant Human CD74 Protein Standard (His tag) (AB315990)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

서열 정보

[{"linker":null,"sequence":"MALLGNFLCCLLVAWLCGPGLGVPLAPADRAPAVGQFWHVTDLHLDPTYHITDDRTKVCASSKGANASNPGPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELSTGTVIKVITNMTMTVQNLFPNLQVFPALGNHDYWPQDQLPIVTSKVYSAVADLWKPWLGEEAISTLKKGGFYSQKVASNPGLRIISLNTNLYYGPNIMTLNKTDPANQFEWLENTLNSSLWNKEKVYIIAHVPVGYLPYATDTPAIRQYYNEKLLDIFRRYSSVIAGQFYGHTHRDSLMVLSDKNGNPLNSVFVAPAVTPVKGVLQKETNNPGVRLFQYKPGDYTLLDMVQYYLNLTEANLKGESNWTLEYVLTQAYSVADLQPKSLYALVQQFATKDSKQFLKYYHYYFVSYDSSATCDQHCKTLQVCAIMNLDSMSYDDCLKQHL","proteinLength":"Full Length","predictedMolecularWeight":"49.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":445,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P70158","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
style
left-column

특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

SMPDL3A also known as sphingomyelin phosphodiesterase acid-like 3A is an enzyme with significant roles in lipid metabolism. It weighs around 47 kDa and primarily breaks down sphingomyelins into phosphocholine and ceramides. SMPDL3A shows expression in numerous tissues including the immune system and kidney cells where it aids cellular functions. It is localized primarily on the membranes and it is also found at increasing levels in pathological conditions.
Biological function summary

The enzyme SMPDL3A significantly impacts cellular lipid regulation and membrane dynamics. It does not typically function as part of a complex but stands out in remodeling membrane-bound lipids. SMPDL3A's ability to degrade sphingomyelins affects signaling pathways by altering ceramide levels influencing cellular response processes like apoptosis and proliferation. Therefore its biological role involves influencing membrane composition and cell signaling pathways that respond to various stimuli.

Pathways

This protein significantly participates in the regulation of the lipid metabolism and sphingolipid signaling pathways. It operates alongside other proteins like ceramidase and sphingosine kinase 1. SMPDL3A alters sphingolipid metabolism which influences the MAPK signaling pathway a pathway critical in cell growth and stress responses. By modulating ceramide levels it indirectly affects downstream interactions and protein activities related to these pathways.

Researchers associate SMPDL3A with conditions like diabetic nephropathy and systemic lupus erythematosus (SLE). In diabetic nephropathy SMPDL3A appears to interfere with kidney cellular responses while altered expression levels are noted in pathological states. In SLE SMPDL3A's expression changes link to immune cell dysfunction. The protein also interacts with others involved in these diseases such as CD95/Fas which influences apoptosis pathways further implicating its role in cellular health and disease progression.
style
left-column

일반 정보

기능

Cyclic-nucleotide phosphodiesterase that acts as a negative regulator of innate immunity by mediating degradation of 2',3'-cGAMP, thereby inhibiting the cGAS-STING signaling (PubMed : 37890481). Specifically linearizes 2',3'-cGAMP into 2'5'-bond pGpA and further hydrolyzes pGpA to produce GpA (By similarity). Also has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP (PubMed : 26792860). Has in vitro activity with p-nitrophenyl-TMP (By similarity). Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates (By similarity). Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine (By similarity). Has in vitro activity with CDP-ethanolamine (By similarity). Does not have sphingomyelin phosphodiesterase activity (By similarity).

서열 유사성

Belongs to the acid sphingomyelinase family.

Post-translational modifications

N-glycosylated.

style
left-column

제품 프로토콜

style
left-column

타겟 정보

Cyclic-nucleotide phosphodiesterase that acts as a negative regulator of innate immunity by mediating degradation of 2',3'-cGAMP, thereby inhibiting the cGAS-STING signaling (PubMed : 37890481). Specifically linearizes 2',3'-cGAMP into 2'5'-bond pGpA and further hydrolyzes pGpA to produce GpA (By similarity). Also has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP (PubMed : 26792860). Has in vitro activity with p-nitrophenyl-TMP (By similarity). Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates (By similarity). Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine (By similarity). Has in vitro activity with CDP-ethanolamine (By similarity). Does not have sphingomyelin phosphodiesterase activity (By similarity).
See full target information Smpdl3a
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com