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AB236329

Recombinant Mouse Thrombospondin 1 protein (His tag)

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Recombinant Mouse Thrombospondin 1 protein (His tag) is a Mouse Fragment protein, in the 19 to 350 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

Tsp1, Thbs1, Thrombospondin-1, Glycoprotein G

1 이미지
SDS-PAGE - Recombinant Mouse Thrombospondin 1 protein (His tag) (AB236329)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse Thrombospondin 1 protein (His tag) (AB236329)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab236329.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Mouse

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Proteins & Peptides

AB54317

Recombinant Human YWHAE protein (Tag Free)

SDS-PAGE - Recombinant Human YWHAE protein (Tag Free) (AB54317)

  • 0
  • 0
Primary Antibodies

AB267388

Anti-Thrombospondin 1 antibody [EPR22927-54]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Multiplex immunohistochemistry - Anti-Thrombospondin 1 antibody [EPR22927-54] (AB267388)

  • 2
  • 1
Assay Kits

AB317551

Mouse Thrombospondin 1 ELISA Kit

SimpleStep

Sandwich ELISA - Mouse Thrombospondin 1 ELISA Kit (AB317551)

  • 0
  • 0
Proteins & Peptides

AB276359

Recombinant Human Transglutaminase 2 protein (His tag)

SDS-PAGE - Recombinant Human Transglutaminase 2 protein (His tag) (AB276359)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"NRIPESGGDNGVFDIFELIGGARRGPGRRLVKGQDLSSPAFRIENANLIPAVPDDKFQDLLDAVWADKGFIFLASLRQMKKTRGTLLAVERKDNTGQIFSVVSNGKAGTLDLSLSLPGKQQVVSVEEALLATGQWKSITLFVQEDRAQLYIDCDKMESAELDVPIQSIFTRDLASVARLRVAKGDVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSATNVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGLSCDELSSMVLELKGLRTIVTTLQDSIRKVTEENRELVSELKRPPLCFHNGVQYKNNEEWTVDSCTECHCQNSVTICK","proteinLength":"Fragment","predictedMolecularWeight":"40.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":350,"aminoAcidStart":19,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P35441","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Thrombospondin 1 also known as TSP1 is a multi-functional glycoprotein involved in cellular processes like adhesion migration and tumorigenesis. It has a molecular weight of approximately 450 kDa. TSP1 is produced by multiple cell types including endothelial cells and fibroblasts and it is widely expressed in the extracellular matrix of both normal and tumor tissues. As a member of the thrombospondin family it interacts with cell receptors like integrins and CD36 to mediate its diverse functions.
Biological function summary

TSP1 is significant in modulating angiogenesis immune response and apoptosis. It acts as an anti-angiogenic factor by inhibiting endothelial cell proliferation and migration. TSP1 is a part of a larger complex where it interacts with other matrix proteins and growth factors to fine-tune cellular behavior within tissues. Through its binding domains it inhibits matrix metalloproteinases helping regulate matrix composition and tissue remodeling.

Pathways

TSP1 influences both the TGF-beta and NF-kB signaling pathways. In the TGF-beta pathway TSP1 activates latent TGF-beta contributing to tissue repair and fibrosis processes. It works synergistically with proteins like SMADs in these pathways. In the NF-kB pathway TSP1 modulates immune responses and inflammatory processes. Its interaction with CD36 and integrins in these pathways underlines its versatile role in cellular signaling.

TSP1 has been investigated in cancer progression and cardiovascular diseases. In cancer TSP1's anti-angiogenic properties suppress tumor vascularization which can slow tumor growth. Aberrant TSP1 expression levels have also been linked to atherosclerosis development as it interacts with endothelial cells and inflammatory molecules like TNF-alpha. In both these contexts TSP1 engages with proteins such as integrins and CD36 to exert its influence on disease progression.
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일반 정보

기능

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions (By similarity). Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis (By similarity). Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis (By similarity). Ligand for receptor CD47 (By similarity). Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure (PubMed : 20610415). Plays a role in endothelial cell senescence, acting via CD47, by increasing the abundance and activation of NADPH oxidase NOX1, and so generating excess ROS (PubMed : 29042481). Inhibits stem cell self-renewal, acting via CD47 signaling, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (PubMed : 23591719). Negatively modulates wound healing, acting via CD47 (PubMed : 18156939). Ligand for receptor CD36 (By similarity). Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting via CD36 (PubMed : 25835637). Plays a role in suppressing angiogenesis, acting, depending on context, via CD36 or CD47 (By similarity). Promotes cellular senescence in a TP53-CDKN1A-RB1 signaling-dependent manner (PubMed : 29042481). Ligand for immunoglobulin-like cell surface receptor SIRPA (By similarity). Involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production, acting via interaction with SIRPA (By similarity). Plays a role in metabolic dysfunction in diet-induced obesity, perhaps acting by exacerbating adipose inflammatory activity; its effects may be mediated, at least in part, through enhanced adipocyte proliferation (PubMed : 23757408). Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (PubMed : 22682248). May be involved in age-related conditions, including metabolic dysregulation, during normal aging (PubMed : 29042481, PubMed : 32679764).

서열 유사성

Belongs to the thrombospondin family.

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제품 프로토콜

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타겟 정보

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions (By similarity). Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis (By similarity). Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis (By similarity). Ligand for receptor CD47 (By similarity). Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure (PubMed : 20610415). Plays a role in endothelial cell senescence, acting via CD47, by increasing the abundance and activation of NADPH oxidase NOX1, and so generating excess ROS (PubMed : 29042481). Inhibits stem cell self-renewal, acting via CD47 signaling, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (PubMed : 23591719). Negatively modulates wound healing, acting via CD47 (PubMed : 18156939). Ligand for receptor CD36 (By similarity). Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting via CD36 (PubMed : 25835637). Plays a role in suppressing angiogenesis, acting, depending on context, via CD36 or CD47 (By similarity). Promotes cellular senescence in a TP53-CDKN1A-RB1 signaling-dependent manner (PubMed : 29042481). Ligand for immunoglobulin-like cell surface receptor SIRPA (By similarity). Involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production, acting via interaction with SIRPA (By similarity). Plays a role in metabolic dysfunction in diet-induced obesity, perhaps acting by exacerbating adipose inflammatory activity; its effects may be mediated, at least in part, through enhanced adipocyte proliferation (PubMed : 23757408). Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (PubMed : 22682248). May be involved in age-related conditions, including metabolic dysregulation, during normal aging (PubMed : 29042481, PubMed : 32679764).
See full target information Thbs1
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Product promise

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