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AB322748

Recombinant Mouse Versican Protein Standard (His tag)

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Recombinant Mouse Versican Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.
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Sandwich ELISA - Recombinant Mouse Versican Protein Standard (His tag) (AB322748)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Mouse Versican Protein Standard (His tag) (AB322748)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Mouse Versican Protein Standard (His tag) (AB322748)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse Versican Protein Standard (His tag) (AB322748)

SDS-PAGE analysis of ab322748 under reducing conditions for 2ug protein.

주요 정보

Purity

>80% SDS-PAGE

발현 시스템

HEK 293 cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

Q62059-1

Animal free

Yes

Carrier free

No

Species

Mouse

보관 버퍼

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

서열 정보

[{"linker":null,"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"38.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q62059","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification His Tag
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False

일반 정보

기능

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

서열 유사성

Belongs to the aggrecan/versican proteoglycan family.

Post-translational modifications

Phosphorylated by FAM20C in the extracellular medium.. Proteolytically cleaved by ADAMTS5 and ADAMTS15 in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration.

제품 프로토콜

타겟 정보

May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
See full target information Vcan

대체 명칭 보기

Cspg2, Vcan, Versican core protein, Chondroitin sulfate proteoglycan core protein 2, Large fibroblast proteoglycan, PG-M, Chondroitin sulfate proteoglycan 2

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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