JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB239542

Recombinant Pectate lyase 1 protein (Tagged)

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Recombinant Pectate lyase 1 protein (Tagged) is a Hesperocyparis arizonica Full Length protein, in the 22 to 367 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

대체 명칭 보기

Pectate lyase 1, Major pollen allergen Cup a 1

3 이미지
Mass Spectrometry - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab239542 could indicate that this peptide derived from E.coli-expressed Cupressus arizonica (Arizona cypress) (Callitropsis arizonica) Pectate lyase 1.

Mass Spectrometry - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab239542 could indicate that this peptide derived from E.coli-expressed Cupressus arizonica (Arizona cypress) (Callitropsis arizonica) Pectate lyase 1.

SDS-PAGE - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Pectate lyase 1 protein (Tagged) (AB239542)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab239542.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

10x His tag N-Terminus Myc tag C-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Hesperocyparis arizonica

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

style
left-column

보완 제품

Proteins & Peptides

AB316439

Recombinant Human BCMA Protein Standard (His tag)

Sandwich ELISA - Recombinant Human BCMA Protein Standard (His tag) (AB316439)

  • 0
  • 0
Proteins & Peptides

AB280753

Recombinant Human PD1 protein

Mass Spectrometry - Recombinant Human PD1 protein (AB280753)

  • 0
  • 0
Proteins & Peptides

AB318258

Recombinant human PKM2 protein (His tag)

Mass Spectrometry - Recombinant human PKM2 protein (His tag) (AB318258)

  • 0
  • 0
Proteins & Peptides

AB224859

Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag)

Mass Spectrometry - Recombinant Influenza A Influenza A Virus Nucleoprotein (His tag) (AB224859)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

서열 정보

[{"linker":null,"sequence":"DNPIDSCWRGDSNWDQNRMKLADCVVGFGSSTMGGKGGEIYTVTSSEDNPVNPTPGTLRYGATREKALWIIFSQNMNIKLQMPLYVAGYKTIDGRGADVHLGNGGPCLFMRKASHVILHGLHIHGCNTSVLGDVLVSESIGVEPVHAQDGDAITMRNVTNAWIDHNSLSDCSDGLIDVTLGSTGITISNNHFFNHHKVMLLGHDDTYDDDKSMKVTVAFNQFGPNAGQRMPRARYGLVHVANNNYDQWNIYAIGGSSNPTILSEGNSFTAPNESYKKEVTKRIGCETTSACANWVWRSTRDAFTNGAYFVSSGKAEDTNIYNSNEAFKVENGNAAPQLTQNAGVVA","proteinLength":"Full Length","predictedMolecularWeight":"42.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":367,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9SCG9","tags":[{"tag":"10x His","terminus":"N-Terminus"},{"tag":"Myc","terminus":"C-Terminus"}]}]
style
left-column

특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pectate lyase 1 also known as PL1 or PecL1 is an enzyme that catalyzes the cleavage of pectate a form of pectin into smaller units through a lyase mechanism. The enzyme exhibits a molecular mass of approximately 40 kDa. Pectate lyase 1 is expressed in various plant tissues particularly in areas undergoing cell wall modification such as elongating and ripening fruits leaf abscission zones and germinating seeds. The presence of this enzyme is significant in the breakdown of pectin found in the plant cell wall matrix.
Biological function summary

The action of Pectate lyase 1 aids in cell wall degradation and softening making it essential in the plant's natural growth and development processes. During processes such as fruit ripening leaf fall and seed germination Pectate lyase 1 loosens the cell walls by breaking down pectin which is a significant component of plant cell walls. Pectate lyase 1 operates in coordination with other cell wall-degrading enzymes such as polygalacturonase within a complex that works synergistically to facilitate plant tissue differentiation and development.

Pathways

The activity of Pectate lyase 1 fits into the cell wall modification and glycosaminoglycan degradation pathways. In the cell wall modification pathway Pectate lyase 1 interacts with proteins like polygalacturonase to dismantle pectate contributing to cell separation and expansion. Additionally the enzyme participates in the glycosaminoglycan degradation pathway highlighting its involvement in polysaccharide breakdown alongside other enzymes ensuring the dynamic nature of plant cell walls to accommodate growth and environmental adaptation.

Disruptions in the expression or activity of Pectate lyase 1 can impact both agricultural and environmental contexts. One such impact is fruit softening where overactivity of this enzyme leads to excessive softening and post-harvest losses. Another disease associated with Pectate lyase 1 is bacterial soft rot where pathogens exploit the enzymatic action of Pectate lyase 1 to invade plant tissues. In these contexts the role of other associated proteins such as cellulase becomes important as they work in tandem with Pectate lyase 1 to accelerate tissue degradation during plant pathogenesis.
style
left-column

일반 정보

기능

Has pectate lyase activity.

서열 유사성

Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

style
left-column

제품 프로토콜

style
left-column

타겟 정보

Has pectate lyase activity.
See full target information Pectate lyase 1
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com