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AB236193

Recombinant Pneumolysin protein (Tagged)

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Recombinant Pneumolysin protein (Tagged) is a Full Length protein, in the 2 to 471 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.
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SDS-PAGE - Recombinant Pneumolysin protein (Tagged) (AB236193)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Pneumolysin protein (Tagged) (AB236193)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis with 5% enrichment gel and 15% separation gel of ab236193.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Animal free

No

Carrier free

No

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325996

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands) (AB325996)

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Primary Antibodies

AB71810

Anti-Pneumolysin antibody [PLY-4]

Recombinant

Western blot - Anti-Pneumolysin antibody [PLY-4] (AB71810)

  • 5
  • 1
Proteins & Peptides

AB240861

Recombinant Pneumolysin protein (His tag)

SDS-PAGE - Recombinant Pneumolysin protein (His tag) (AB240861)

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Proteins & Peptides

AB52953

Recombinant Staphylococcus aureus Protein A

Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (AB52953)

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Proteins & Peptides

AB225646

Recombinant Human HLA B protein (His tag)

Mass Spectrometry - Recombinant Human HLA B protein (His tag) (AB225646)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"ANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFVVIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSIDLPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQLKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTVTVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQTEWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVLTPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRTISIWGTTLYPQVEDKVEND","proteinLength":"Full Length","predictedMolecularWeight":"68.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":471,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Pneumolysin also known as Ply or ply-004 is a toxin protein produced by Streptococcus pneumoniae a bacterium responsible for many infections. It has a molecular mass of approximately 53 kDa. Pneumolysin is a cholesterol-dependent cytolysin meaning that it targets and binds to cholesterol in host cell membranes leading to pore formation and cell lysis. The toxin is predominantly expressed in S. pneumoniae where it plays an essential role in pathogenicity.
Biological function summary

Pneumolysin affects host cells by disrupting cellular integrity and immune responses. It is not part of a larger protein complex. Pneumolysin can inhibit cilia movement in the respiratory tract impairing the host’s ability to clear mucus and pathogens. This toxin also activates the host immune system by promoting the release of pro-inflammatory cytokines which can result in tissue damage.

Pathways

Pneumolysin plays a significant role in the inflammatory response and cell apoptosis pathways. Within the inflammatory pathway it can trigger the host's immune cells to release cytokines such as interleukin-6 and tumor necrosis factor-alpha. This response leads to increased vascular permeability and leukocyte migration. Pneumolysin-mediated cell lysis also contributes to apoptosis impacting cell survival. The toxin's action relates it to proteins involved in the immune signaling cascades.

Pneumolysin plays a vital role in diseases such as pneumonia and meningitis both caused by S. pneumoniae. The disruption of host cell membranes and induction of inflammation contribute directly to disease symptoms. Pneumolysin interacts with proteins involved in the host's immune defense during infection. These interactions and its cytotoxic properties make pneumolysin a target for research into vaccine and therapeutic strategies against pneumococcal diseases.
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일반 정보

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제품 프로토콜

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타겟 정보

See full target information Pneumolysin

Additional targets

Pneumolysin
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Product promise

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