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AB226274

Recombinant Pseudomonas aeruginosa Beta-lactamase protein (His tag)

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Recombinant Pseudomonas aeruginosa Beta-lactamase protein (His tag) is a Pseudomonas aeruginosa PAO1 Full Length protein, in the 27 to 397 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

대체 명칭 보기

PA4110, ampC, Beta-lactamase, Cephalosporinase, ampA, ampC, Cephalosporinase

1 이미지
SDS-PAGE - Recombinant Pseudomonas aeruginosa Beta-lactamase protein (His tag) (AB226274)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Pseudomonas aeruginosa Beta-lactamase protein (His tag) (AB226274)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226274 with 5% enrichment gel and 15% separation gel.

주요 정보

Purity

>90% SDS-PAGE

발현 시스템

Escherichia coli

Tags

6x His tag N-Terminus SUMO tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Pseudomonas aeruginosa PAO1

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Assay Kits

AB197008

Beta Lactamase Activity Assay Kit (Colorimetric)

Functional Studies - Beta Lactamase Activity Assay Kit (Colorimetric) (AB197008)

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Proteins & Peptides

AB226272

Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag)

Mass Spectrometry - Recombinant E. coli Beta-lactamase CTX-M-1 protein (His tag) (AB226272)

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Primary Antibodies

AB12251

Anti-Beta Lactamase antibody [8A5.A10]

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Proteins & Peptides

AB106879

Recombinant E. coli hchA protein (His tag N-Terminus)

SDS-PAGE - Recombinant E. coli hchA protein (His tag N-Terminus) (AB106879)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"GEAPADRLKALVDAAVQPVMKANDIPGLAVAISLKGEPHYFSYGLASKEDGRRVTPETLFEIGSVSKTFTATLAGYALTQDKMRLDDRASQHWPALQGSRFDGISLLDLATYTAGGLPLQFPDSVQKDQAQIRDYYRQWQPTYAPGSQRLYSNPSIGLFGYLAARSLGQPFERLMEQQVFPALGLEQTHLDVPEAALAQYAQGYGKDDRPLRVGPGPLDAEGYGVKTSAADLLRFVDANLHPERLDRPWAQALDATHRGYYKVGDMTQGLGWEAYDWPISLKRLQAGNSTPMALQPHRIARLPAPQALEGQRLLNKTGSTNGFGAYVAFVPGRDLGLVILANRNYPNAERVKIAYAILSGLEQQGKVPLKR","proteinLength":"Full Length","predictedMolecularWeight":"56.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":397,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P24735","tags":[{"tag":"6x His","terminus":"N-Terminus"},{"tag":"SUMO","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
False
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Beta-lactamase often referred to as penicillinase is a protein that catalyzes the hydrolysis of beta-lactam antibiotics like penicillins. This enzyme breaks the beta-lactam ring structure rendering the antibiotic ineffective. Beta-lactamase proteins vary widely in their molecular mass with most commonly falling in the range of 29 to 35 kDa. These enzymes are predominantly expressed in bacteria including 'E. coli' where they confer antibiotic resistance. The production of beta-lactamase can occur at the periplasmic space in gram-negative bacteria or secreted in gram-positive bacteria.
Biological function summary

Beta-lactamase enzymes cause antibiotic resistance which is a significant concern in treating bacterial infections. These proteins function as monomeric enzymes but may work with other factors or be part of larger resistance mechanisms. They degrade beta-lactam antibiotics protecting the bacteria from antibacterial agents. In 'E. coli' beta-lactamase production is upregulated in the presence of antibiotics showcasing an adaptive bacterial response.

Pathways

Beta-lactamase activity integrates into bacterial defense mechanisms particularly through antibiotic resistance pathways. The enzyme plays an important role in the broader antibiotic resistance pathway working alongside other resistance proteins such as penicillin-binding proteins (PBPs) which alter the target sites of antibiotics. The production and function of these proteins ensure the survival of resistant bacteria even in antibiotic-rich environments.

Beta-lactamase enzymes are closely linked to challenging bacterial infections particularly in nosocomial (hospital-acquired) infections. Their presence complicates the treatment of diseases like pneumonia and urinary tract infections (UTIs) often making standard antibiotics ineffective. Beta-lactamase is associated with multidrug-resistant strains like extended-spectrum beta-lactamase (ESBL) producing bacteria which can complicate treatment options and outcomes. Understanding this protein and its interactions with other proteins like efflux pumps is important in addressing antibiotic resistance.
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일반 정보

기능

Class C beta-lactamase which confers resistance to penicillins and cephalosporins (By similarity). Has nitrocefin-hydrolyzing activity (PubMed : 16251297).

서열 유사성

Belongs to the class-C beta-lactamase family.

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제품 프로토콜

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타겟 정보

Class C beta-lactamase which confers resistance to penicillins and cephalosporins (By similarity). Has nitrocefin-hydrolyzing activity (PubMed : 16251297).
See full target information ampC
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Product promise

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