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AB282395

Recombinant rat IL-4 protein (Active)

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Recombinant rat IL-4 protein (Active) is a Rat Full Length protein, in the 25 to 147 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC.

대체 명칭 보기

Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

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Functional Studies - Recombinant rat IL-4 protein (Active) (AB282395)
  • FuncS

Supplier Data

Functional Studies - Recombinant rat IL-4 protein (Active) (AB282395)

Functional analysis of ab282395.

Fully biologically active determined by dose dependent increase in proliferation of rat splenocytes.

ED50 for this effect is ≤ 0.66 ng/ml corresponding to specific activity of 1.51 X 106 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot : GR3393276-4

Mass Spectrometry - Recombinant rat IL-4 protein (Active) (AB282395)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant rat IL-4 protein (Active) (AB282395)

Mass determination by ESI-TOF.

Predicted MW is 13820.00 Da (+/- 10 Da by ESI-TOF). Observed MW is 13821.71

SDS-PAGE - Recombinant rat IL-4 protein (Active) (AB282395)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant rat IL-4 protein (Active) (AB282395)

SDS-page analysis of ab282395.

HPLC - Recombinant rat IL-4 protein (Active) (AB282395)
  • HPLC

Supplier Data

HPLC - Recombinant rat IL-4 protein (Active) (AB282395)

HPLC analysis of ab282395.

주요 정보

Purity

>95% HPLC

Endotoxin 레벨

<0.005 EU/µg

발현 시스템

HEK 293 cells

Tags

Tag free

Applications

FuncS, HPLC, SDS-PAGE, Mass Spec

applications

Biologically active

Yes

Biological activity

Fully biologically active determined by dose dependent increase in proliferation of rat splenocytes.

ED50 for this effect is ≤ 0.66 ng/ml corresponding to specific activity of 1.51 X 106 units/mg.

Accession

Animal free

Yes

Carrier free

No

Species

Rat

Reconstitution

Reconstitute in PBS

보관 버퍼

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Potassium phosphate monobasic

storage-buffer

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보완 제품

Reagents, Controls & Accessories

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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서열 정보

[{"linker":null,"sequence":"CNDSPLREIINTLNQVTEKGTPCTEMFVPDVLTATRNTTENELICRASRVLRKFYFPRDVPPCLKNKSGVLGELRKLCRGVSGLNSLRSCTVNESTLTTLKDFLESLKSILRGKYLQSCTSMS","proteinLength":"Full Length","predictedMolecularWeight":"13.82 kDa","actualMolecularWeight":null,"aminoAcidEnd":147,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P20096","tags":[]}]
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특성 및 보관 정보

제형
Lyophilized
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
True
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일반 정보

기능

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.

서열 유사성

Belongs to the IL-4/IL-13 family.

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제품 프로토콜

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타겟 정보

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information Il4
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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