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AB240857

Recombinant Rat Trypsin protein

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Recombinant Rat Trypsin protein is a Rat Full Length protein, in the 24 to 247 aa range, expressed in Yeast, with >85%, suitable for SDS-PAGE.

대체 명칭 보기

Trypsin-4, P23, Pretrypsinogen IV, Trypsin IV

1 이미지
SDS-PAGE - Recombinant Rat Trypsin protein (AB240857)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Rat Trypsin protein (AB240857)

Analysis of ab240857 by (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

주요 정보

Purity

>85% SDS-PAGE

발현 시스템

Yeast

Tags

6x His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

Animal free

No

Carrier free

No

Species

Rat

보관 버퍼

pH: 7.2 - 7.4 Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

서열 정보

[{"linker":null,"sequence":"IVGGYTCPKHLVPYQVSLHDGISHQCGGSLISDQWVLSAAHCYKRKLQVRLGEHNIHVLEGGEQFIDAEKIIRHPEYNKDTLDNDIMLIKLKSPAVLNSQVSTVSLPRSCASTDAQCLVSGWGNTVSIGGKYPALLQCLEAPVLSASSCKKSYPGQITSNMFCLGFLEGGKDSCDGDSGGPVVCNGEIQGIVSWGSVCAMRGKPGVYTKVCNYLSWIQETMANN","proteinLength":"Full Length","predictedMolecularWeight":"26.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":247,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Yeast","accessionNumber":"P12788","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]

특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
False

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Trypsin is a serine protease enzyme with a molecular mass around 24 kDa. Known also as "bovine trypsin" or "pancreatic trypsin" it functions primarily in the digestive system. Trypsin hydrolyzes peptide bonds specifically at the carboxyl side of the amino acids lysine and arginine. The enzyme is produced in the pancreas as an inactive precursor trypsinogen which converts to active trypsin in the small intestine.
Biological function summary

Trypsin breaks down proteins into smaller peptides facilitating digestion. It is not part of a complex but interacts with other pancreatic enzymes like chymotrypsin and elastase. Trypsin also plays a regulatory role in activating other zymogens like procarboxypeptidase and chymotrypsinogen essential in the complete protein digestion process.

Pathways

The catalytic actions of trypsin are integral to the digestive pathway in the gastrointestinal tract. It ensures the efficient breakdown and absorption of nutrients. In the trypsin activation pathway it activates other proteases which are significant for protein catabolism. This enzyme collaborates closely with anti-trypsin inhibitors that regulate its activity preventing autodigestion and maintaining homeostasis.

Trypsin relates closely to pancreatitis and cystic fibrosis. Unregulated activation of trypsinogen within the pancreas causes pancreatitis a condition characterized by inflammation and damage to pancreatic cells. Alpha-1 anti-trypsin an important inhibitor of trypsin connects to conditions like emphysema and liver disease due to its dysfunctional regulation indicating a broader implication of trypsin in maintaining cellular health.

일반 정보

기능

Serine protease capable of autoactivation.

서열 유사성

Belongs to the peptidase S1 family.

Post-translational modifications

Proteolytically cleaved and activated by an autocatalytic mechanism (By similarity). Cleavage by CTRC inhibits autoactivation (By similarity).

제품 프로토콜

타겟 정보

Serine protease capable of autoactivation.
See full target information Try4

Product promise

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