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AB89494

Recombinant S. japonicum GST protein (Active) (His tag N-Terminus)

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(3 제품이 사용된 논문 )

Recombinant S. japonicum GST protein (Active) (His tag N-Terminus) is a Schistosoma japonicum Full Length protein, in the 1 to 218 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.
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SDS-PAGE - Recombinant S. japonicum GST protein (Active) (His tag N-Terminus) (AB89494)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant S. japonicum GST protein (Active) (His tag N-Terminus) (AB89494)

3ug of reduced ab89494 on SDS-PAGE, stained with Coomassie Blue.

주요 정보

Purity

>95% SDS-PAGE

ab89494 is purified using conventional chromatography techniques.

발현 시스템

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 10 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25°C

Accession

P08515-1

Animal free

No

Carrier free

No

Species

Schistosoma japonicum

보관 버퍼

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

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Primary Antibodies

AB111947

Anti-GST antibody [EPR4236]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GST antibody [EPR4236] (AB111947)

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Primary Antibodies

AB229327

Anti-LC3B antibody - N-terminal

Lab Essentials

Flow Cytometry - Anti-LC3B antibody - N-terminal (AB229327)

  • 0
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Primary Antibodies

AB317397

Anti-POT1 antibody [S06-9I1]

Recombinant

  • 0
  • 0
Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

MW confirmed by MALDI-TOF.
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서열 정보

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPR","proteinLength":"Full Length","predictedMolecularWeight":"28.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":218,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P08515","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GST or Glutathione S-transferase is an enzyme family known for detoxifying endogenous and exogenous compounds through conjugation with glutathione. GSTs are about 23–28 kDa in size and are expressed in various tissues such as the liver kidneys and lungs. The GST proteins play vital roles in cellular processes and are utilized extensively in molecular biology often tagged to proteins to enhance solubility known as GST-tagged proteins.
Biological function summary

GST enzymes participate in critical cellular detoxification processes enhancing the solubility of toxins. As part of these processes GST enzymes do not function as part of a larger complex but are individual actors within the cell. They facilitate the conjugation reactions fundamental to cellular metabolism and detoxification making them essential for maintaining cellular health.

Pathways

GST enzymes contribute significantly to the metabolic breakdown pathways like the xenobiotic metabolism pathway. This pathway involves proteins such as cytochrome P450 enzymes which often function upstream to modify compounds for further GST action. GSTs are important for efficiently detoxifying potentially harmful substances preventing cellular damage.

GSTs have considerable relevance to cancer where their overexpression can confer drug resistance in tumor cells complicating treatment approaches. Additionally these enzymes connect with proteins such as MDR1 influencing multidrug resistance in cancer therapy. GSTs also relate to asthma where altered GST activity can affect the body's response to oxidative stress highlighting the enzyme's role in inflammatory processes.
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일반 정보

기능

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.

서열 유사성

Belongs to the GST superfamily. Mu family.

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제품 프로토콜

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타겟 정보

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.. GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut.
See full target information Glutathione S-transferase class-mu 26 kDa isozyme

대체 명칭 보기

Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

World journal of gastrointestinal oncology 16:1006-1018 PubMed38577450

2024

Ubiquitin-specific protease 21 promotes tumorigenicity and stemness of colorectal cancer by deubiquitinating and stabilizing ZEB1.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jun Lin,Ye-Cai Lu

Nature communications 14:4930 PubMed37582753

2023

Diversity-oriented synthesis encoded by deoxyoligonucleotides.

Applications

Unspecified application

Species

Unspecified reactive species

Liam Hudson,Jeremy W Mason,Matthias V Westphal,Matthieu J R Richter,Jonathan R Thielman,Bruce K Hua,Christopher J Gerry,Guoqin Xia,Heather L Osswald,John M Knapp,Zher Yin Tan,Praveen Kokkonda,Ben I C Tresco,Shuang Liu,Andrew G Reidenbach,Katherine S Lim,Jennifer Poirier,John Capece,Simone Bonazzi,Christian M Gampe,Nichola J Smith,James E Bradner,Connor W Coley,Paul A Clemons,Bruno Melillo,C Suk-Yee Hon,Johannes Ottl,Christoph E Dumelin,Jonas V Schaefer,Ann Marie E Faust,Frédéric Berst,Stuart L Schreiber,Frédéric J Zécri,Karin Briner

Nature communications 13:6707 PubMed36344525

2022

SAMHD1 deacetylation by SIRT1 promotes DNA end resection by facilitating DNA binding at double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Kapoor-Vazirani,Sandip K Rath,Xu Liu,Zhen Shu,Nicole E Bowen,Yitong Chen,Ramona Haji-Seyed-Javadi,Waaqo Daddacha,Elizabeth V Minten,Diana Danelia,Daniela Farchi,Duc M Duong,Nicholas T Seyfried,Xingming Deng,Eric A Ortlund,Baek Kim,David S Yu
제품이 사용된 논문 모두 보기
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Product promise

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