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AB60884

Recombinant Src protein (GST tag N-Terminus)

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(5 제품이 사용된 논문 )

Recombinant Src protein (GST tag N-Terminus) is a Full Length protein, expressed in Baculovirus infected Sf9 cells, with >90%, suitable for SDS-PAGE, FuncS.
4 이미지
Functional Studies - Recombinant Src protein (GST tag N-Terminus) (AB60884)
  • FuncS

Unknown

Functional Studies - Recombinant Src protein (GST tag N-Terminus) (AB60884)

Sample Kinase Activity Plot.

Functional Studies - Recombinant Src protein (GST tag N-Terminus) (AB60884)
  • FuncS

Unknown

Functional Studies - Recombinant Src protein (GST tag N-Terminus) (AB60884)

The specific activity of Src (ab60884) was determined to be 112 nmol/min/mg as per activity assay protocol

SDS-PAGE - Recombinant Src protein (GST tag N-Terminus) (AB60884)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Src protein (GST tag N-Terminus) (AB60884)

ab60884 on SDS-PAGE, MW ~85 kDa.

SDS-PAGE - Recombinant Src protein (GST tag N-Terminus) (AB60884)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Src protein (GST tag N-Terminus) (AB60884)

SDS PAGE analysis of ab60884

주요 정보

Purity

>90% SDS-PAGE

Purity: >90% as determined by densitometry. Affinity purified.

발현 시스템

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

116nmol/min/mg

Animal free

No

Carrier free

No

보관 버퍼

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0038% EGTA, 0.00292% EDTA, 0.00174% PMSF

storage-buffer

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보완 제품

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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  • 0
Proteins & Peptides

AB51277

Recombinant human PTP1B protein (Active) (Tag Free)

SDS-PAGE - Recombinant human PTP1B protein (Active) (Tag Free) (AB51277)

  • 0
  • 0
Proteins & Peptides

AB60844

Recombinant human PKC delta protein (GST tag N-Terminus)

Western blot - Recombinant human PKC delta protein (GST tag N-Terminus) (AB60844)

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Proteins & Peptides

AB155812

Recombinant human ERK2 protein (GST tag N-Terminus)

Functional Studies - Recombinant human ERK2 protein (GST tag N-Terminus) (AB155812)

  • 0
  • 0
Assay Kits

AB139486

Aggresome Detection Kit

Fluorescence Microscopy - Aggresome Detection Kit (AB139486)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

ab204883 (CDK1 peptide) can be utilized as a substrate for assessing kinase activity
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서열 정보

[{"linker":null,"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFMGSSKSKPKDPSQRRRSLEPPDSTHHGGFPASQTPNKTAAPDTHRTPSRSFGTVATEPKLFGDFNTSDTVTSPQRAGALAGGVTTFVALYDYESWIETDLSFKKGERLQIVNNTEGNWWLAHSVTTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSSLQQLVAYYSKHADGLCHRLTNVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVIEYMSKGSLLDFLKGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRMPYPGMGNGEVLDRVERGYRMPCPPECPESLHDLMCQCWRRDPEERPTFEYLQAQLLPACVLEVAE","proteinLength":"Full Length","predictedMolecularWeight":"85 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":null,"tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-80°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
True
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Src also known as c-Src is a protein-tyrosine kinase involved in the regulation of many cellular processes. The molecular weight of Src is approximately 60 kDa. Src is ubiquitously expressed in human tissues but shows increased expression in specific tissues like the brain and epithelial cells. This protein has several important roles in cellular signal transduction particularly influencing cell growth differentiation and survival.
Biological function summary

The Src protein interacts with other proteins to modulate cell adhesion motility and angiogenesis forming part of larger protein complexes. Src phosphorylates specific tyrosine residues on its substrates altering their activity interaction or stability. This activity positions Src as an important factor in managing cell communication and structural organization. Src's interaction with focal adhesion complexes emphasizes its functionality in cellular structural integrity and intracellular communication pathways.

Pathways

Src plays a critical role in the integrin and growth factor receptor signaling pathways mediating cross-talk between cell surface receptors and intricate signaling cascades. It closely associates with focal adhesion kinase (FAK) within these pathways influencing cytoskeletal rearrangements and cell movement. Src's function in the epidermal growth factor receptor (EGFR) signaling pathway likewise demonstrates its importance in regulating cellular proliferation and survival mechanisms.

Src has significant implications in oncogenesis particularly in colorectal and breast cancers. Overexpression or abnormal activity of Src associates with tumor progression and metastasis. Within these cancers Src cooperates with various proteins like the EGFR amplifying aberrant cell signaling that contributes to uncontrolled cell growth. Investigating Src's role and regulation could offer insights into novel therapeutic strategies for controlling Src activity in cancer treatment.
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일반 정보

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제품 프로토콜

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타겟 정보

See full target information SRC
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Autophagy 20:505-524 PubMed37772772

2023

Direct regulation of FNIP1 and FNIP2 by MEF2 sustains MTORC1 activation and tumor progression in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Xia,Tiejian Nie,Fangfang Lu,Lu Huang,Xiaolong Shi,Dongni Ren,Jianjun Lu,Xiaobin Li,Tuo Xu,Bozhou Cui,Qing Wang,Guodong Gao,Qian Yang

Analytical biochemistry 608:113892 PubMed32810472

2020

Electrochemical detection of neuronal extracellular phosphorylation by PKA, PKC and Src.

Applications

Unspecified application

Species

Unspecified reactive species

S Ahmad,M N Hossain,S Ahmadi,K Kerman,H-B Kraatz

Analytical biochemistry 574:46-56 PubMed30914244

2019

Electrochemical studies of human nAChR a7 subunit phosphorylation by kinases PKA, PKC and Src.

Applications

Unspecified application

Species

Unspecified reactive species

Syed Ahmad,Zhe She,Heinz-Bernhard Kraatz

Nature communications 9:2590 PubMed29968724

2018

Postprandial FGF19-induced phosphorylation by Src is critical for FXR function in bile acid homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Sangwon Byun,Dong-Hyun Kim,Daniel Ryerson,Young-Chae Kim,Hao Sun,Bo Kong,Peter Yau,Grace Guo,H Eric Xu,Byron Kemper,Jongsook Kim Kemper

The Journal of biological chemistry 292:2065-2079 PubMed27994061

2016

Focal Adhesion Kinase-mediated Phosphorylation of Beclin1 Protein Suppresses Cardiomyocyte Autophagy and Initiates Hypertrophic Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaokang Cheng,Qiang Zhu,Rachel Dee,Zachary Opheim,Christopher P Mack,Douglas M Cyr,Joan M Taylor
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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