AB241023

Albumin Depletion Kit

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(2 제품이 사용된 논문 )

Albumin Depletion Kit ab241023 provides easy and user-friendly spin columns for the depletion/removal of abundant albumin protein from samples for downstream applications.

- Uses Cibacron blue immobilized on beads for albumin binding
- Each spin column can bind ~2-3 mg albumin
- Produce ready-to- use albumin-depleted samples in 60 minutes

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Functional Studies - Albumin Depletion Kit (AB241023)

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Supplier Data

Functional Studies - Albumin Depletion Kit (AB241023)

Depletion of albumin from plasma using ab241023

Samples were analyzed by 15% SDS-PAGE. M : Protein Marker, 1 : Eluted albumin fraction (20 μL of concentrated eluted albumin, primarily containing albumin), 2 : Albumin-depleted plasma sample (20 μL of combined and concentrated flow-through and wash fractions).

주요 정보

샘플 타입

Purified protein

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제품 세부 정보

Albumin Depletion Kit (ab241023) provides easy and user-friendly spin columns for the depletion/removal of abundant albumin protein from samples thus enhancing the sample analysis. The kit is based on the interaction of Cibacron Blue 3G-A (immobilized on beads) with albumin. Each spin column can bind ~2-3 mg of albumin and processes samples in less than one hour. After albumin depletion, samples can be used for subsequent ELISA, immunoassays and other downstream analyses such as gel electrophoresis and functional assays. These columns can also be used for purification and enrichment of a variety of Cibacron Blue dye-binding proteins.

Please note, this kit has only been tested on human samples.

This product is manufactured by BioVision, an Abcam company and was previously called K6573 EZAlbumin™ Depletion Kit (Spin Column). K6573-25 is the same size as the 25 test size of ab241023.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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제품 구성

{ "values": { "25Test": { "sellingSize": "25 Test", "publicAssetCode":"ab241023-25Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Cibacron Elution Buffer", "number":"AB241023-CMP03", "productcode":"" }, { "size":"1 x 33 mL", "name":"Albumin Binding Buffer", "number":"AB241023-CMP01", "productcode":"" }, { "size":"1 x 25 Vial", "name":"Cibacron Blue Column", "number":"AB241023-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.

Biological function summary

Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.

Pathways

More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.

Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.

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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 14:9104 PubMed38643249

2024

SMS121, a new inhibitor of CD36, impairs fatty acid uptake and viability of acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah Åbacka,Samuele Masoni,Giulio Poli,Peng Huang,Francesco Gusso,Carlotta Granchi,Filippo Minutolo,Tiziano Tuccinardi,Anna K Hagström-Andersson,Karin Lindkvist-Petersson

Nature protocols 18:1462-1487 PubMed36914899

2023

Comprehensive isolation of extracellular vesicles and nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Zhang,Dennis K Jeppesen,James N Higginbotham,Jeffrey L Franklin,Robert J Coffey

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Albumin Depletion Kit

Functional Studies - Albumin Depletion Kit (AB241023)

주요 정보

샘플 타입

보완 제품

Secondary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise