Antibody Purification Kit (Protein A)

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Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. Antibody Purification Kit (Protein A) (ab102784) quickly removes these contaminants. It can also purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up is ideally in a volume of 100-500 µl.

Up to 20-500 µg of antibody can be purified in each run.

The method involves capture of the antibody on Protein A resin and the removal of unwanted substances by a simple wash procedure, which is carried out in a standard microfuge. The purified product is then eluted and neutralized.

Note: This method cannot be used with samples containing relatively dilute antibody in large volumes (e.g. tissue culture supernatant). For larger volumes, we recommend the use of Antibody TCS Purification Kit (3 purifications) (ab109207) or Antibody Serum Purification kit (3 purifications) (ab109209).

Antibodies purified using the Antibody Purification Kit (Protein A) are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our GOLD Antibody conjugation kits. To purify antibodies for use with our Gold conjugation kits, please use our Gold antibody purification kit (ab204909)

ab102784 is not suitable for goat antibody purification. Please refer to the protocol booklet for more information about suitable and unsuitable species antibodies.

Abcam's antibody purification and concentration kitsoffer you a streamlined and efficient solution for isolating and concentrating antibodies from various sources. These kits are designed with simplicity and effectiveness in mind, allowing you to achieve high-purity antibodies with minimal hands-on time. Using our advanced purification technologies, we ensure that your antibodies retain their functionality and specificity, making them ideal for a wide range of applications, including western blot, immunohistochemistry (IHC), and ELISA.

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Learn about our Lightning-Link® antibody and Oligonucleotide labeling kits and their advantages here.