Phalloidin-iFluor 790 Reagent

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Phalloidin-iFluor 790 Reagent (ab176763) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 790 can be detected with a fluorescent microscope at Ex/Em = 787/808 nm (infrared range).

Each 300 test product includes 1 vial containing 30 µg of iFluor 790-dye-conjugated-phalloidin. Add in 30 µL of DMSO to make a concentration of 1 mg/mL. We recommend using this at a 1:1000 dilution, see protocol booklet for more instructions.

Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.

Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the dissociation rate of actin subunits from filaments ends, essentially stabilizing actin filaments through the prevention of filament depolymerization.

The vial containing ab176763 may appear to be empty. The packaging process involves aliquoting the compound dissolved in solvent and subsequent evaporation. This results in the deposition of a thin film of material that may not be clearly visible.

Reagent Preparation

Before opening, briefly centrifuge the small vial at low speed to ensure all contents are collected at the bottom and to prevent loss of material.