VeriBlot for IP Detection Reagent (HRP)

• IP

Supplier Data

All lanes:

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (<a href='/ko/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a>) at 1/500 dilution

Lane 1:

Jurkat whole cell lysate (input) at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> at 1/30 IP in Jurkat whole cell lysate (+) at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> in Jurkat whole cell lysate (-) at 10 µg

Predicted band size: 37 kDa

true

Exposure time: 10s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

CD5 was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate with ab325643 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325643 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 2 : ab325643 IP in MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325643 in MOLT-4 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 76 seconds.

All lanes:

Immunoprecipitation - Anti-CD5 antibody [RM1352] (<a href='/ko/products/primary-antibodies/cd5-antibody-rm1352-ab325643'>ab325643</a>) at 1/1000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/cd5-antibody-rm1352-ab325643'>ab325643</a> at 1/30 IP in MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/cd5-antibody-rm1352-ab325643'>ab325643</a> in MOLT-4 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 67 kDa

false

Exposure time: 76s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Ret was immunoprecipitated from 0.35 mg TT (human thyroid carcinoma epithelial cell) treated with mM pervanadate for 30 min whole cell lysate with ab326572 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326572 at 1/326572 1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : TT (human thyroid carcinoma epithelial cell) treated with 1mM pervanadate for 30 min whole cell lysate
Lane 2 : ab326572 IP in TT (human thyroid carcinoma epithelial cell) treated with mM pervanadate for 30 min whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326572 in TT (human thyroid carcinoma epithelial cell) treated with 1mM pervanadate for 30 min whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Ret (phospho Y1062) antibody [EPR30639-507] (<a href='/ko/products/primary-antibodies/ret-phospho-y1062-antibody-epr30639-507-ab326572'>ab326572</a>) at 1/5000 dilution

Lane 1:

TT (human thyroid carcinoma epithelial cell) treated with 1mM pervanadate for 30 min whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/ret-phospho-y1062-antibody-epr30639-507-ab326572'>ab326572</a> at 1/30 IP in TT (human thyroid carcinoma epithelial cell) treated with mM pervanadate for 30 min whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/ret-phospho-y1062-antibody-epr30639-507-ab326572'>ab326572</a> in TT (human thyroid carcinoma epithelial cell) treated with 1mM pervanadate for 30 min whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 150 kDa

false

Exposure time: 10s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

NELFe was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab326107 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326107 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : ab326107 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326107 in K-562 lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds.

To minimize protein degradation, cells/tissues(lane 2, lane 4, lanes 5-8) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Immunoprecipitation - Anti-NELFe antibody [EPR29850-562] (<a href='/ko/products/primary-antibodies/nelfe-antibody-epr29850-562-ab326107'>ab326107</a>) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/nelfe-antibody-epr29850-562-ab326107'>ab326107</a> at 1/30 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/nelfe-antibody-epr29850-562-ab326107'>ab326107</a> in K-562 lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 43 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

ALK (pY1586) was immunoprecipitated from 0.35 mg KARPAS-299 (human T cell lymphoma cell) treated with 50uM pervanadate for 30min, whole cell lysate 10ug with ab305097 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab305097 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : KARPAS-299 (human T cell lymphoma cell) treated with 50uM pervanadate for 30min, whole cell lysate 10ug

Lane 2 : ab305097 IP in KARPAS-299 treated with 50uM pervanadate for 30min, whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab305097 in KARPAS-299 treated with 50uM pervanadate for 30min, whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

Lysate was freshly made and used for IP immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-ALK(pY1586) antibody [EPR26900-88] (<a href='/ko/products/primary-antibodies/alkpy1586-antibody-epr26900-88-ab305097'>ab305097</a>) at 1/1000 dilution

All lanes:

<a href='/ko/products/primary-antibodies/alkpy1586-antibody-epr26900-88-ab305097'>ab305097</a> IP in KARPAS-299 treated with 50uM pervanadate for 30min, whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 80 kDa

Observed band size: 80 kDa

false

Exposure time: 32s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Musashi 1 / MSI1 was immunoprecipitated from HAP1 (human chronic myelogenous leukemia near-haploid cell line) whole cell lysate 10 μg with ab305170 1/1000 dilution. Western blot was performed on the immunoprecipitate using ab305170 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates).

Rabbit monoclonal IgG (ab172730) Isotype Control was used instead of ab305170 in HAP1 whole cell lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Musashi 1 / MSI1 antibody [EPR26106-92] (<a href='/ko/products/primary-antibodies/musashi-1-msi1-antibody-epr26106-92-ab305170'>ab305170</a>) at 1/1000 dilution

All lanes:

HAP1 human chronic myelogenous leukemia near-haploid cell line) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 39 kDa

false

Exposure time: 50s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

TNF alpha was immunoprecipitated from 0.35 mg of TPA pretreated THP-1 (Human monocytic leukemia cell line) whole cell lysate treated with 100 ng/ml LPS for 4 hours, then added 1 μg/ml BFA for 3 hours with ab183218 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183218 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1 : TPA pretreated THP-1 whole cell lysate treated with 100 ng/ml LPS for 4 hours, then added 1 μg/ml BFA for 3 hours 10µg (Input). Lane 2 : ab183218 IP in TPA pretreated THP-1 whole cell lysate treated with 100 ng/ml LPS for 4 hours, then added 1 μg/ml BFA for 3 hours. Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab183218 in TPA pretreated THP-1 whole cell lysate treated with 100 ng/ml LPS for 4 hours, then added 1 μg/ml BFA for 3 hours. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-TNF alpha antibody [EPR19147] (<a href='/ko/products/primary-antibodies/tnf-alpha-antibody-epr19147-ab183218'>ab183218</a>)

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

false

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Nurr1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) was starved for 2 hours, and then treated with 200 nM PMA for 2 hours, whole cell lysate with ab327018 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab327018 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) was starved for 2 hours, and then treated with 200 nM PMA for 2 hours, whole cell lysate
Lane 2 : ab327018 IP in HeLa (human cervical adenocarcinoma epithelial cell) was starved for 2 hours, and then treated with 200 nM PMA for 2 hours, whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab327018 in HeLa was starved for 2 hours, and then treated with 200 nM PMA for 2 hours, whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 50 seconds.

All lanes:

Immunoprecipitation - Anti-Nurr1 antibody [EPR26338-73] (<a href='/ko/products/primary-antibodies/nurr1-antibody-epr26338-73-ab327018'>ab327018</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) was starved for 2 hours, and then treated with 200 nM PMA for 2 hours, whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/nurr1-antibody-epr26338-73-ab327018'>ab327018</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) was starved for 2 hours, and then treated with 200 nM PMA for 2 hours, whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/nurr1-antibody-epr26338-73-ab327018'>ab327018</a> in HeLa was starved for 2 hours, and then treated with 200 nM PMA for 2 hours, whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 60 kDa,67 kDa

false

Exposure time: 50s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RYBP was immunoprecipitated from HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg with ab185971 1/1000 dilution. Western blot was performed on the immunoprecipitate using ab185971 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates).

Rabbit monoclonal IgG (ab172730) Isotype Control was used instead of ab185971 in HEK-293 whole cell lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-RYBP antibody [EPR13059(2)] - ChIP Grade (<a href='/ko/products/primary-antibodies/rybp-antibody-epr130592-chip-grade-ab185971'>ab185971</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate (Low Expression Sample) at 10 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 32 kDa

false

Exposure time: 15s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Progranulin was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell fresh lysate with ab327420 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab327420 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell fresh lysate
Lane 2 : ab327420 IP in 293T (human embryonic kidney epithelial cell) whole cell fresh lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab327420 in 293T whole cell fresh lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

The band beneath the target band (74 kDa) is likely to be isoform of Progranulin. This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-Progranulin antibody [MJF-D22103-42R] (<a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell fresh lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a> at 1/30 IP in 293T (human embryonic kidney epithelial cell) whole cell fresh lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a> in 293T whole cell fresh lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 74 kDa

true

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SH3MD2 was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma epithelial cell) fresh whole cell lysate with ab326568 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326568 at 1/326568 1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell) fresh whole cell lysate
Lane 2 : ab326568 IP in HCT 116 (human colorectal carcinoma epithelial cell) fresh whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326568 in HCT 116 fresh whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 145 seconds.

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-SH3MD2 antibody [EPR30521-594] (<a href='/ko/products/primary-antibodies/sh3md2-antibody-epr30521-594-ab326568'>ab326568</a>) at 1/1000 dilution

Lane 1:

HCT 116 (human colorectal carcinoma epithelial cell) fresh whole cell lysate at 20 µg

Lane 2:

<a href='/ko/products/primary-antibodies/sh3md2-antibody-epr30521-594-ab326568'>ab326568</a> at 1/30 IP in HCT 116 (human colorectal carcinoma epithelial cell) fresh whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/sh3md2-antibody-epr30521-594-ab326568'>ab326568</a> in HCT 116 fresh whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 145s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

GSDMD was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab323316 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323316 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-GSDMD antibody [EPR23078-13R] (<a href='/ko/products/primary-antibodies/gsdmd-antibody-epr23078-13r-ab323316'>ab323316</a>) at 1/1000 dilution

Lane 1:

(Input) PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/gsdmd-antibody-epr23078-13r-ab323316'>ab323316</a> at 1/30 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/gsdmd-antibody-epr23078-13r-ab323316'>ab323316</a> in PC-3 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 52 kDa

false

Exposure time: 10s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

AGO2(phospho S387) was immunoprecipitated from 0.35 mg HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate with ab326569 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326569 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate
Lane 2 : ab326569 IP in HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326569 in HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-AGO2(phospho S387) antibody [EPR29226-168] (<a href='/ko/products/primary-antibodies/ago2phospho-s387-antibody-epr29226-168-ab326569'>ab326569</a>) at 1/1000 dilution

Lane 1:

HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/ago2phospho-s387-antibody-epr29226-168-ab326569'>ab326569</a> at 1/30 IP in HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/ago2phospho-s387-antibody-epr29226-168-ab326569'>ab326569</a> in HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 97 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

PIWIL4/PIWI was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab324050 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324050 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5%

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Immunoprecipitation - Anti-PIWIL4/PIWI antibody [EPR28186-10] (<a href='/ko/products/primary-antibodies/piwil4-piwi-antibody-epr28186-10-ab324050'>ab324050</a>) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/piwil4-piwi-antibody-epr28186-10-ab324050'>ab324050</a> at 1/30 IP in K-562 whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/piwil4-piwi-antibody-epr28186-10-ab324050'>ab324050</a> in K-562 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 96 kDa

false

Exposure time: 111s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RIP3 was immunoprecipitated from 0.35 mg HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab325636 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325636 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab325636 IP in HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325636 in HT-29 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-RIP3 antibody [RM1355] (ab325636) at 1/1000 dilution

Lane 1:

HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

ab325636 at 1/30 IP in HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab325636 in HT-29 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 37-56 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Dnmt3a was immunoprecipitated from 0.35 mg Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab323708 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323708 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Dnmt3a antibody [EPR29184-89] (<a href='/ko/products/primary-antibodies/dnmt3a-antibody-epr29184-89-ab323708'>ab323708</a>) at 1/1000 dilution

Lane 1:

(Input) Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/dnmt3a-antibody-epr29184-89-ab323708'>ab323708</a> at 1/30 IP in Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/dnmt3a-antibody-epr29184-89-ab323708'>ab323708</a> in parental HeLa whole cell lysate at 10 µg

Lane 4:

(Input) DNMT3A knockout HeLa whole cell lysate at 10 µg

Lane 5:

<a href='/ko/products/primary-antibodies/dnmt3a-antibody-epr29184-89-ab323708'>ab323708</a> at 1/30 IP in DNMT3A knockout HeLa whole cell lysate at 10 µg

Lane 6:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/dnmt3a-antibody-epr29184-89-ab323708'>ab323708</a> in DNMT3A knockout HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 24s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Estrogen Related Receptor alpha was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab327347 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab327347 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : ab327347 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab327347 in K-562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR30313-532] (ab327347) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

Lane 2:

ab327347 at 1/30 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab327347 in K-562 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 50 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Cyclin E1 was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma epithelial cell) treated with 2.5mM Thymidine for 16h whole cell lysate with ab327426 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab327426 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HCT 116 (human colorectal carcinoma epithelial cell) treated with 2.5mM Thymidine for 16h whole cell lysate
Lane 2 : ab327426 IP in HCT 116 (human colorectal carcinoma epithelial cell) treated with 2.5mM Thymidine for 16h whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab327426 in HCT 116 treated with 2.5mM Thymidine for 16h whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-Cyclin E1(Phospho T77) antibody [EPR28889-10] (ab327426) at 1/1000 dilution

Lane 1:

HCT 116 (human colorectal carcinoma epithelial cell) treated with 2.5mM Thymidine for 16h whole cell lysate at 10 µg

Lane 2:

ab327426 at 1/30 IP in HCT 116 (human colorectal carcinoma epithelial cell) treated with 2.5mM Thymidine for 16h whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab327426 in HCT 116 treated with 2.5mM Thymidine for 16h whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 35-50 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

HDAC9 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab326719 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326719 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : ab326719 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326719 in SH-SY5Y whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-HDAC9 antibody [EPR30363-35] (<a href='/ko/products/primary-antibodies/hdac9-antibody-epr30363-35-ab326719'>ab326719</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/hdac9-antibody-epr30363-35-ab326719'>ab326719</a> at 1/30 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/hdac9-antibody-epr30363-35-ab326719'>ab326719</a> in SH-SY5Y whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 90 kDa,150 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

GSDMD was immunoprecipitated from 0.35 mg Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate with ab323316 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323316 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-GSDMD antibody [EPR23078-13R] (<a href='/ko/products/primary-antibodies/gsdmd-antibody-epr23078-13r-ab323316'>ab323316</a>) at 1/1000 dilution

Lane 1:

(Input) Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/gsdmd-antibody-epr23078-13r-ab323316'>ab323316</a> at 1/30 IP in Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/gsdmd-antibody-epr23078-13r-ab323316'>ab323316</a> in Jurkat whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 52 kDa

false

Exposure time: 10s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

MED12 was immunoprecipitated from 0.35 mg LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab325642 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325642 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab325642 IP in LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325642 in LoVo whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-MED12 antibody [EPR29665-541] (<a href='/ko/products/primary-antibodies/med12-antibody-epr29665-541-ab325642'>ab325642</a>) at 1/1000 dilution

Lane 1:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/med12-antibody-epr29665-541-ab325642'>ab325642</a> at 1/30 IP in LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/med12-antibody-epr29665-541-ab325642'>ab325642</a> in LoVo whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 270 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

NFATC4 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab324108 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324108 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-NFATC4 antibody [EPR25678-90] (<a href='/ko/products/primary-antibodies/nfatc4-antibody-epr25678-90-ab324108'>ab324108</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/nfatc4-antibody-epr25678-90-ab324108'>ab324108</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/nfatc4-antibody-epr25678-90-ab324108'>ab324108</a> in HUVEC whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Observed band size: 80-150 kDa

false

Exposure time: 67s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

UBP43/USP18 was immunoprecipitated from 0.35 mg Wide-type A549 treated with 100U/ml IFN alpha (human) for 14 hours whole cell lysate with ab325178 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325178 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-UBP43/USP18 antibody [EPR26174-540] (<a href='/ko/products/primary-antibodies/ubp43-usp18-antibody-epr26174-540-ab325178'>ab325178</a>) at 1/1000 dilution

Lane 1:

Wide-type A549 treated with 100U/ml IFN alpha (human) for 14 hours whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/ubp43-usp18-antibody-epr26174-540-ab325178'>ab325178</a> at 1/30 IP in Wide-type A549 treated with 100U/ml IFN alpha (human) for 14 hours whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/ubp43-usp18-antibody-epr26174-540-ab325178'>ab325178</a> in Wide-type A549 treated with 100U/ml IFN alpha (human) for 14 hours whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 35 kDa,37 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Tet2 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell fresh lysate with ab324875 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324875 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

The bands beneath the target band (260 kDa) are likely to be degraded target fragments.
To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-Tet2 antibody [EPR29630-29] (<a href='/ko/products/primary-antibodies/tet2-antibody-epr29630-29-ab324875'>ab324875</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell fresh lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/tet2-antibody-epr29630-29-ab324875'>ab324875</a> at 1/30 IP in 293T (human embryonic kidney epithelial cell) whole cell fresh lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/tet2-antibody-epr29630-29-ab324875'>ab324875</a> in 293T whole cell fresh lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 260 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SMARCA2 / BRM was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab323179 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323179 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-SMARCA2 / BRM antibody [EPR28611-92] (<a href='/ko/products/primary-antibodies/smarca2-brm-antibody-epr28611-92-ab323179'>ab323179</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/smarca2-brm-antibody-epr28611-92-ab323179'>ab323179</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/smarca2-brm-antibody-epr28611-92-ab323179'>ab323179</a> in HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 200 kDa

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

KMT3A / HYPB / HIF-1 was immunoprecipitated from 0.35 mg ab324102 at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab324102 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324102 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-KMT3A / HYPB / HIF-1 antibody [EPR28172-44] (<a href='/ko/products/primary-antibodies/kmt3a-hypb-hif-1-antibody-epr28172-44-ab324102'>ab324102</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 2:

<a href='/ko/products/primary-antibodies/kmt3a-hypb-hif-1-antibody-epr28172-44-ab324102'>ab324102</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/kmt3a-hypb-hif-1-antibody-epr28172-44-ab324102'>ab324102</a> in Hela whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 120-350 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RIP3 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab325636 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325636 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate at
Lane 2 : ab325636 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316957 in THP-1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 50 seconds.

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-RIP3 antibody [RM1355] (ab325636) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at at 10 µg

Lane 2:

ab325636 at 1/30 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/rip3-antibody-epr24374-9-ab316957'>ab316957</a> in THP-1 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 37-60 kDa

false

Exposure time: 50s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Progranulin was immunoprecipitated from 0.35 mg HEK293T (human embryonic kidney epithelial cell) whole cell lysate with ab327420 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab327420 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The band beneath the target band (74 kDa) in lane 2 is likely to be isoform of Progranulin.

All lanes:

Immunoprecipitation - Anti-Progranulin antibody [MJF-D22103-42R] (<a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a> in parental HEK293T whole cell lysate

Lane 4:

Progranulin knockout HEK293T whole cell lysate at 10 µg

Lane 5:

Progranulin knockout HEK293T whole cell lysate

Lane 6:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a> in DNMT3A knockout HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Observed band size: 74 kDa

false

Exposure time: 84s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

GPR89a was immunoprecipitated from 0.35 mg T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate with ab325319 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325319 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-GPR89a antibody [EPR29290-504] (<a href='/ko/products/primary-antibodies/gpr89a-antibody-epr29290-504-ab325319'>ab325319</a>) at 1/1000 dilution

Lane 1:

(Input) T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate

Lane 2:

<a href='/ko/products/primary-antibodies/gpr89a-antibody-epr29290-504-ab325319'>ab325319</a> at 1/30 IP in T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/gpr89a-antibody-epr29290-504-ab325319'>ab325319</a> in T-47D whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Observed band size: 35 kDa

false

Exposure time: 15s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SNX2 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab326422 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326422 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab326422 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326422 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds.

All lanes:

Immunoprecipitation - Anti-SNX2 antibody [EPR30535-531] (<a href='/ko/products/primary-antibodies/snx2-antibody-epr30535-531-ab326422'>ab326422</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/snx2-antibody-epr30535-531-ab326422'>ab326422</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/snx2-antibody-epr30535-531-ab326422'>ab326422</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 72 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Transportin 1/MIP was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab324273 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324273 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Transportin 1/MIP antibody [EPR29606-11] (<a href='/ko/products/primary-antibodies/transportin-1-mip-antibody-epr29606-11-ab324273'>ab324273</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/transportin-1-mip-antibody-epr29606-11-ab324273'>ab324273</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/transportin-1-mip-antibody-epr29606-11-ab324273'>ab324273</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 102 kDa

false

Exposure time: 3s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

BACH1.3 was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab324640 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324640 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : ab324640 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab324640 in K-562 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds.

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-BACH1.3 antibody [EPR29608-726] (<a href='/ko/products/primary-antibodies/bach13-antibody-epr29608-726-ab324640'>ab324640</a>) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/bach13-antibody-epr29608-726-ab324640'>ab324640</a> at 1/30 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/bach13-antibody-epr29608-726-ab324640'>ab324640</a> in K-562 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 24s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Rad9 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate with ab327458 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab327458 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : ab327458 IP in 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab327458 in 293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 67 seconds.

All lanes:

Immunoprecipitation - Anti-Rad9 antibody [EPR30495-553] (<a href='/ko/products/primary-antibodies/rad9-antibody-epr30495-553-ab327458'>ab327458</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/rad9-antibody-epr30495-553-ab327458'>ab327458</a> at 1/30 IP in 293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/rad9-antibody-epr30495-553-ab327458'>ab327458</a> in 293T whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 60 kDa

false

Exposure time: 67s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SA2 (isoform-1) was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate with ab324732 at 1/30 dilution (2 ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324732 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-SA2 (isoform-1) antibody [EPR30102-55] (<a href='/ko/products/primary-antibodies/sa2-isoform-1-antibody-epr30102-55-ab324732'>ab324732</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/sa2-isoform-1-antibody-epr30102-55-ab324732'>ab324732</a> at 1/30 IP in 293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/sa2-isoform-1-antibody-epr30102-55-ab324732'>ab324732</a> in 293T whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 140 kDa

false

Exposure time: 3s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

BATF was immunoprecipitated from 0.35 mg HDLM-2 (human hodgkin lymphoma cell) whole cell lysate with ab324487 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324487 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HDLM-2 (human hodgkin lymphoma cell) whole cell lysate
Lane 2 : ab324487 IP in HDLM-2 (human hodgkin lymphoma cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab324487 in HDLM-2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-BATF antibody [EPR28945-705] (ab324487) at 1/1000 dilution

Lane 1:

HDLM-2 (human hodgkin lymphoma cell) whole cell lysate at 10 µg

Lane 2:

ab324487 at 1/30 IP in HDLM-2 (human hodgkin lymphoma cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab324487 in HDLM-2 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 17 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SA2 (isoform-1) was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab324732 at 1/30 dilution (2 ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324732 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-SA2 (isoform-1) antibody [EPR30102-55] (<a href='/ko/products/primary-antibodies/sa2-isoform-1-antibody-epr30102-55-ab324732'>ab324732</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/sa2-isoform-1-antibody-epr30102-55-ab324732'>ab324732</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/sa2-isoform-1-antibody-epr30102-55-ab324732'>ab324732</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 140 kDa

false

Exposure time: 3s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

eNOS was immunoprecipitated from HUVEC (human umbilical vein endothelial cell) whole cell lysate 10 μg with ab199956 1/1000 dilution. Western blot was performed on the immunoprecipitate using ab199956 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates).

Rabbit monoclonal IgG (ab172730) Isotype Control was used instead of ab199956 in HUVEC (human umbilical vein endothelial cell) whole cell lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-eNOS antibody [EPR19296] (<a href='/ko/products/primary-antibodies/enos-antibody-epr19296-ab199956'>ab199956</a>) at 1/1000 dilution

All lanes:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 140 kDa

false

Exposure time: 6s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RILPL2 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab325436 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325436 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : ab325436 at 1/30 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325436 in THP-1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 137 seconds.

All lanes:

Immunoprecipitation - Anti-RILPL2 antibody [MJF-D29763-69] (<a href='/ko/products/primary-antibodies/rilpl2-antibody-mjf-d29763-69-ab325436'>ab325436</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/rilpl2-antibody-mjf-d29763-69-ab325436'>ab325436</a> at 1/30 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/rilpl2-antibody-mjf-d29763-69-ab325436'>ab325436</a> in THP-1 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 26 kDa

false

Exposure time: 137s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Lane 1 : Wild-type HEK-293 input Lane 2 : HOXA9 knockout HEK-293 input Lane 3 : Wild-type HEK-293 immunoprecipitated with anti-HOXA9 antibody ab140631 Lane 4 : HOXA9 knockout HEK-293 immunoprecipitated with anti-HOXA9 antibody ab140631 Lane 5 : Wild-type HEK-293 immunoprecipitated with isotype control antibody ab172730 Lane 6 : HOXA9 knockout HEK-293 immunoprecipitated with isotype control antibody ab172730 HOXA9 was immunoprecipitated with Recombinant Anti-HOXA9 antibody [EPR3655(2)] (ab140631) at 10 µg ml-1. A non-specific band detected in the input material at 37 kDa was not immunoprecipitated. ab172730 was used as the isotype control. Western blot was performed on immunoprecipitates of wild-type and HOXA9 knockout HEK-293 cell lysates (ab273706). To generate this image, wild-type and HOXA9 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with VeriBlot for IP Detection Reagent (HRP) (ab131366) for 1 h at room temperature, washed again four times then imaged.

All lanes:

Immunoprecipitation - Anti-HOXA9 antibody [EPR3655(2)] (<a href='/ko/products/primary-antibodies/hoxa9-antibody-epr36552-ab140631'>ab140631</a>) at 10 µg/mL

Lane 1:

Wild-type HEK-293 input

Lane 2:

HOXA9 knockout HEK-293 input

Lane 3:

Wild-type HEK-293 immunoprecipitated with anti-HOXA9 antibody <a href='/ko/products/primary-antibodies/hoxa9-antibody-epr36552-ab140631'>ab140631</a>

Lane 4:

HOXA9 knockout HEK-293 immunoprecipitated with anti-HOXA9 antibody <a href='/ko/products/primary-antibodies/hoxa9-antibody-epr36552-ab140631'>ab140631</a>

Lane 5:

Wild-type HEK-293 immunoprecipitated with isotype control antibody <a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>

Lane 6:

HOXA9 knockout HEK-293 immunoprecipitated with isotype control antibody <a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366)

Predicted band size: 30 kDa

false

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Progranulin was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell) whole cell fresh lysate with ab327420 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab327420 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : A431 (human epidermoid carcinoma epithelial cell) whole cell fresh lysate
Lane 2 : ab327420 IP in A431 (human epidermoid carcinoma epithelial cell) whole cell fresh lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab327420 in A431 whole cell fresh lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 58 seconds.

The band beneath the target band (74 kDa) is likely to be isoform of Progranulin.

All lanes:

Immunoprecipitation - Anti-Progranulin antibody [MJF-D22103-42R] (<a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a>) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell fresh lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a> at 1/30 IP in A431 (human epidermoid carcinoma epithelial cell) whole cell fresh lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/progranulin-antibody-mjf-d22103-42r-ab327420'>ab327420</a> in A431 whole cell fresh lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 74 kDa

false

Exposure time: 58s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

PNPLA3 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab326041 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326041 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : ab326041 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326041 in HepG2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-PNPLA3 antibody [EPR29816-94] (<a href='/ko/products/primary-antibodies/pnpla3-antibody-epr29816-94-ab326041'>ab326041</a>) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/ko/products/primary-antibodies/pnpla3-antibody-epr29816-94-ab326041'>ab326041</a> at 1/30 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/pnpla3-antibody-epr29816-94-ab326041'>ab326041</a> in HepG2 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 70 kDa

false

Exposure time: 10s