JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB282569

Western Blot Stripping Buffer

Be the first to review this product! 리뷰 제출

|

(3 제품이 사용된 논문 )

Western Blot Stripping Buffer (ab282569) uniformly removes antibodies from Western blots so blots may be reprobed with different antibodies in just 25 minutes.

- No harsh reagents and does not damage antigens or membranes
- Compatible with nitrocellulose and PVDF membranes
2 이미지
Western blot - Western Blot Stripping Buffer (AB282569)
  • WB

Supplier Data

Western blot - Western Blot Stripping Buffer (AB282569)

Western Blot Stripping Buffer strips antibodies with minimal antigen loss.

Untreated HeLa cell lysate (4, 2,1 and 0.5 μg) was separated by SDS-PAGE and transfered to either nitrocellulose or PVDF membranes. Blots were probed with an aAnti-GAPDH antibody (Panel A). After stripping with Western Blot Stripping Buffer, no residual signal was detected (Panel B). The blots were then re-probed with an Anti-Stat1 antibody (Panel C).

false

Western blot - Western Blot Stripping Buffer (AB282569)
  • WB

Supplier Data

Western blot - Western Blot Stripping Buffer (AB282569)

Untreated HeLa cell lysate (4, 2,1 and 0.5 μg) was separated by SDS-PAGE and transfered to either nitrocellulose or PVDF membranes. Blots were probed with an Anti-GAPDH antibody (Panel A). After stripping with Western Blot Stripping Buffer, no residual signal was detected (Panel B). The blots were then re-probed with an Anti-Stat1 antibody (Panel C).

Similar results were obtained with chemilumiescent reagents from 2 other manufacturers.

false

주요 정보

제형

Liquid

form

보관 버퍼

pH: 2.9 - 3.1 Constituents: 88% Water, 10% Sodium lauryl sulfate

storage-buffer

style
left-column

보완 제품

Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

KD Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
Secondary Antibodies

AB205719

Goat Anti-Mouse IgG H&L (HRP)

Western blot - Goat Anti-Mouse IgG H&L (HRP) (AB205719)

  • 5
  • 4
style
left-column

제품 세부 정보

Western Blot Stripping Buffer (ab282569) uniformly removes antibodies from Western blots so that the blots may be reprobed with different antibodies. Western Blot Stripping Buffer is compatible with nitrocellulose and PVDF membranes.

Stripping and reprobing saves precious samples. The entire protocol takes just 25 minutes and removes bound antibodies without harsh reagents or elevated temperatures; it will not damage antigens or membranes.

Western Blot Stripping Buffer is compatible with most chemiluminescent reagents.

We also offer the Western Blot Marker Pen (ab166858) for blot annotation.

style
left-column

특성 및 보관 정보

배송 시 보관 조건
Ambient - Cannot Ship with Ice
적절한 단기 보관 조건
Ambient
적절한 장기 보관 조건
Ambient
style
left-column
style
left-column
style
left-column

제품 프로토콜

style
left-column

타겟 정보

style
left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록and refine your search

International journal of molecular sciences 25: PubMed39456979

2024

Mangiferin Represses Inflammation in Macrophages Under a Hyperglycemic Environment Through Nrf2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ravichandran Jayasuriya,Kumar Ganesan,Kunka Mohanram Ramkumar

Journal of clinical pharmacology 64:1193-1203 PubMed38831713

2024

Sodium-Glucose Cotransporter-2 Inhibitor Suppresses Endoplasmic Reticulum Stress and Oxidative Stress in Diabetic Nephropathy Through Nrf2 Signaling: A Clinical and Experimental Study.

Applications

Unspecified application

Species

Unspecified reactive species

Murali Krishna Prasad,Paul S Victor,Goutham V Ganesh,Udayama Juttada,Satyavani Kumpatla,Vijay Viswanathan,Kunka Mohanram Ramkumar

Stem cell research & therapy 15:66 PubMed38443965

2024

Amelioration of diabetic nephropathy in mice by a single intravenous injection of human mesenchymal stromal cells at early and later disease stages is associated with restoration of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing He,Boxin Liu,Xiaofeng Du,Yan Wei,Desheng Kong,Baofeng Feng,Ruiyun Guo,Ernest Amponsah Asiamah,Matthew D Griffin,Sean O Hynes,Sanbing Shen,Yan Liu,Huixian Cui,Jun Ma,Timothy O'Brien
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com