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AB267478

Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9)

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Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (ab267478) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.
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Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)
  • Flow Cyt

Supplier Data

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Cell culture exosomes, pre-enriched using Total Exosome Isolation from PC3 Cell Culture Media (A), were resuspended in PBS and bound to CD63-capture beads during an overnight incubation. The following day the bead-bound exosomes were direct stained with primary detection antibody (CD9-PE) and analyzed by flow cytometry.

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)
  • Flow Cyt

Supplier Data

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Relationship between background noise and specific signal at different exosome concentrations.

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)
  • Flow Cyt

Supplier Data

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (CD63 / CD9) (AB267478)

Dot-plot gating strategy for acquisition and analysis. FSC vs SSC (A) and PerCP vs APC (B)

주요 정보

검출 방식

Fluorescent

분석 플랫폼

Flow cytometer

샘플 타입

Cell culture media

Reacts with

Human

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보완 제품

Sample Prep & Detection Kits

AB267479

Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81)

Flow Cytometry - Exosome Isolation and Analysis Kit - Flow Cytometry, Plasma (CD9 / CD81) (AB267479)

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Primary Antibodies

AB133615

Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB133615)

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Sample Prep & Detection Kits

AB64264

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Primary Antibodies

AB236630

Anti-CD9 antibody [EPR23105-121]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-121] (AB236630)

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제품 세부 정보

Exosome Isolation and Analysis Kit - Flow Cytometry, Cell culture (ab267478) is a simple immunobead assay for isolation/detection of pre-enriched CD63+ human exosomes from biofluids (plasma, urine) or cell culture media.

Using a bead-bound anti-CD63 capture antibody and a fluorochrome conjugated anti-CD9 detection antibody, the kit provides reproducible results and can be run in parallel to exosome immunophenotyping.

The kit and the measurement of exosomes is useful in the identification of apoptotic cells by flow cytometry.

Visit our FAQs page for tips and troubleshooting.

Exosomes are small extracellular vesicles that are released from cells upon fusion of an intermediate endocytic compartment, the multivesicular body (MVB), with the plasma membrane. They are thought to provide a means of intercellular communication and of transmission of macromolecules between cells allowing the spread of proteins, lipids, mRNA, miRNA and DNA and as contributing factors in the development of several diseases. Exosomes can also modulate cancer microenvironment and the immune response.

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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
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적절한 장기 보관 조건
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보관 정보
+4°C
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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Saudi dental journal 36:1425-1431 PubMed39619718

2024

The potential of exosomes from adipose-derived stromal-vascular fraction in Increasing Migration Activity of Human Dental Pulp Stromal Cells (in vitro ).

Applications

Unspecified application

Species

Unspecified reactive species

Sylva Dinie Alinda,Anggraini Margono,Indah Yulianto,Ike Dwi Maharti,Reizka Asadelia Rafmawan

BMC immunology 23:37 PubMed35941539

2022

Blockade of exosome generation by GW4869 inhibits the education of M2 macrophages in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yilin Peng,Min Zhao,Yinying Hu,Hongyan Guo,Yanyan Zhang,Yanqin Huang,Lin Zhao,Yong Chai,Zhigang Wang
제품이 사용된 논문 모두 보기
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Product promise

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