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AB288085

Fraction-PREP Cell Fractionation Kit

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(2 제품이 사용된 논문 )

Fraction-PREP Cell Fractionation Kit ab288085 is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.

주요 정보

샘플 타입

Suspension cells, Tissue Homogenate, Adherent cells

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보완 제품

Sample Prep & Detection Kits

AB109719

Cell Fractionation Kit - Standard

Western blot - Cell Fractionation Kit - Standard (AB109719)

  • 5
  • 5
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
Assay Kits

AB14085

Annexin V-FITC Apoptosis Staining / Detection Kit

Functional Studies - Annexin V-FITC Apoptosis Staining / Detection Kit (AB14085)

  • 5
  • 3
Reagents, Controls & Accessories

AB176753

Phalloidin-iFluor 488 Reagent

Immunocytochemistry/ Immunofluorescence - Phalloidin-iFluor 488 Reagent (AB176753)

  • 5
  • 14
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제품 세부 정보

Fraction-PREP Cell Fractionation Kit (ab288085), previously known as FractionPREP™ Cell Fractionation Kit (Cat# K270-50), is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.

This product is manufactured by BioVision, an Abcam company and was previously called K270 FractionPREP™ Cell Fractionation Kit. K270-50 is the same size as the 50 test size of ab288085.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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제품 구성

{ "values": { "50Test": { "sellingSize": "50 Test", "publicAssetCode":"ab288085-50Test", "assetComponentDetails": [ { "size":"1 x 1.2 mL", "name":"Lysis Buffer X", "number":"AB288085-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"Extraction Buffer VI", "number":"AB288085-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"Extraction Buffer V", "number":"AB288085-CMP06", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Protease Inhibitor Cocktail I", "number":"AB288085-CMP02", "productcode":"" }, { "size":"1 x 150 µL", "name":"DTT Stock (1M)", "number":"AB288085-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"Extraction Buffer III", "number":"AB288085-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Please refer to protocols
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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2404961 PubMed39932384

2025

Ribonuclease 1 Induces T-Cell Dysfunction and Impairs CD8 T-Cell Cytotoxicity to Benefit Tumor Growth through Hijacking STAT1.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hao Yang,Bao-Yue Huang,Hsing-Yu Rao,Peng Ye,Bi Chen,Hao-Ching Wang,Chih-Hung Chung,Heng-Hsiung Wu,Hung-Rong Yen,Shao-Chun Wang,Jong-Ho Cha,Xiuwen Yan,Muh-Hwa Yang,Mien-Chie Hung

Cancers 16: PubMed39061185

2024

Anticancer Effect of PtPHEN, Pt5ME, Pt56ME and Their Platinum(IV)-Dihydroxy Derivatives against Triple-Negative Breast Cancer and Cisplatin-Resistant Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Maria George Elias,Shadma Fatima,Timothy J Mann,Shawan Karan,Meena Mikhael,Paul de Souza,Christopher P Gordon,Kieran F Scott,Janice R Aldrich-Wright
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com