Human CD4+ T Cell Isolation Kit delivers a highly enriched population of healthy, viable CD4+ T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.
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Flow Cytometry - Human CD4+ T Cell Isolation Kit (AB322033)
Untouched human CD4+ T cells were isolated from MNCs using the Human CD4+ T Cell Isolation Kit. Isolated cells were labeled with CD3-FITC and CD4-APC. The fluorescently labeled cells were analyzed by flow cytometry. Dead cells/debris were excluded from analysis.
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Human CD4+ T Cell Isolation Kit delivers a highly enriched population of healthy, viable CD4+ T cells using a protocol that is fast, easy, and exceptionally gentle on delicate immune cells.
- This kit is designed to process 10 samples, up to 100 million cells per sample
- Fast & easy workflow enriches human peripheral blood mononuclear cells (PBMCs) for CD4+ T cells in 30 minutes
- No magnet, no column, no harsh chemicals
- Microbubble enrichment maintains cell health and physiology
Mechanism of Action: Human CD4+ T Cell Isolation Kit uses a negative selection method to remove unwanted cell populations from the sample, effectively enriching for the T cells of interest while maintaining cellular physiology. The undesired (non-CD4+ T) cells are labeled with an optimized cocktail of biotinylated antibodies targeting CD8, CD11b, CD14, CD16, CD19, CD36, CD56, CD123, CD235a, and TCR-γδ.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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