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AB325298

Alpaca monoclonal [14A4] Anti-Mouse IgG2a/b antibody (AZdye 568)

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Alpaca monoclonal [14A4] Anti-Mouse IgG2a/b antibody (AZdye 568) (ab325298) is a ready-to-go fluorescent camelid single-domain antibody (sdAb, nanobody, VHH) conjugate with maximum absorption wavelength of 578 nm and a maximum emission wavelength of 602 nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF applications.

  • Quantitative and reliable readout in fluorescence-based analyses
  • Defined number of fluorophores enhance brightness of signals
  • Improved localization accuracy ideal for super resolution imaging
  • Designed to recognize conventional immunoglobulins with high isotype specificity
1 이미지
Immunocytochemistry/ Immunofluorescence - Alpaca monoclonal [14A4] Anti-Mouse IgG2a/b antibody (AZdye 568) (AB325298)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alpaca monoclonal [14A4] Anti-Mouse IgG2a/b antibody (AZdye 568) (AB325298)

Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilized, quenched with 0.1M ammonium chloride, and blocked with 10% normal goat serum in PBS HeLa (human cervical cancer cells) cells labelling beta Tubulin with ab315214 at 1 : 2000 (0.50 µg/ml) dilution, followed by ab325298 Alpaca monoclonal [14A4] Anti-Mouse IgG2a/b antibody (AZdye 568) at 1/500 (0.15 µg/ml per sdAb) dilution (red).

Confocal image showing cytoplasmic staining in HeLa cells (shown in red). The counterstain was observed in yellow. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a fluorescence microscope (Zeiss Axio Imager Z1).

ab92742 anti-Ki67 antibody was used to counterstain Ki67 at 1/1000 (0.85 µg/ml) dilution (yellow).

Secondary antibody only control : Secondary antibody is ab325298 Alpaca monoclonal [14A4] Anti-Mouse IgG2a/b antibody (AZdye 568) at 1/500 (0.15 µg/ml per sdAb) dilution.

주요 정보

Host species

Alpaca

Target species

Mouse

Target isotype

IgG2a, IgG2b

Target specificity

Heavy chain hinge

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

AZdye 568

Excitation/Emission

Ex: 578nm, Em: 602nm

Applications

ICC/IF

applications

Clonality

Monoclonal

Clone number

14A4

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500", "notes":"<p></p>" } } }

제품 세부 정보

Together with their monovalent binding mode, the defined fluorophore conjugation of ab325298 results in a quantitative and reliable readout for several fluorescence-based applications.  It contains a defined number of fluorophores at known positions. In addition due to the small size the fluorophore displacement from the bound target protein is very low making ab325298 ideal for standard super resolution technologies.

Features:
- High affinity monovalent binding + two fluorophores per sdAb for brighter signals.
- Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
In comparison to conventional secondary reagents the ab325298 nanobody has the following advantages:
- Small probes for enhanced staining speed and improved tissue penetration and higher resolution.
- Monoclonal tools with defined species  and isotype specificity.
- Recombinant production for reliable quality, scalability and reproducibility.
- Monovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible.
- Enhanced binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing).
- Suitable for post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.).

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Size-exclusion chromatography
보관 버퍼
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1 month
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-80°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

타겟 정보

See full target information Ighg

대체 명칭 보기

Immunoglobulin heavy chain gamma polypeptide, Ighg
websiteProtocolBooklet
en
websiteProtocolBooklet
en

Product promise

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