Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed
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(3 제품이 사용된 논문 )
Suitable for IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
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Ig gamma-1 chain C region
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150152)
ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1μg/ml) overnight at +4°C. The secondary antibody (orange) was ab150152, Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed, used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The secondary antibody only control displays negative staining, as expected.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150152)
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Purification 테크닉
Purification 관련 사항
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
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분주 정보
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제품이 사용된 논문 (3)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Nature communications 15:796 PubMed38280858
2024
Applications
Unspecified application
Species
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Cellular physiology and biochemistry : internation 48:1492-1504 PubMed30071537
2018
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Species
Unspecified reactive species
Nature biotechnology 34:973-81 PubMed27454740
2016
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Unspecified application
Species
Unspecified reactive species
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