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AB7120

Donkey Anti-Goat IgG H&L preadsorbed

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(4 제품이 사용된 논문 )

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.
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ELISA - Donkey Anti-Goat IgG H&L preadsorbed (AB7120)
  • ELISA

Supplier Data

ELISA - Donkey Anti-Goat IgG H&L preadsorbed (AB7120)

ELISA results of ab7120 against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 µg of Goat IgG as well as Chicken IgG, Guinea Pig IgG, Hamster IgG, Horse IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1/56,000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated, and TMB substrate.

주요 정보

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster

Pre-adsorbed

Yes

Applications

IHC-P, ELISA, WB, Conjugation, IP, IHC-Fr, ID, ICC/IF

applications

Clonality

Polyclonal

Specificity

<p>No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins. Specificity was confirmed using ELISA minimal cross reactivity against the targets stated above.</p>

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보완 제품

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Secondary Antibodies

AB182021

Donkey Anti-Goat IgG H&L

ELISA - Donkey Anti-Goat IgG H&L (AB182021)

  • 0
  • 0
Secondary Antibodies

AB97081

Donkey Anti-Rabbit IgG H&L preadsorbed

Western blot - Donkey Anti-Rabbit IgG H&L preadsorbed (AB97081)

  • 0
  • 0
Secondary Antibodies

AB150129

Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (AB150129)

  • 5
  • 6
Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/150000 - 1/250000", "notes":"<p></p>" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
보관 버퍼
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

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타겟 정보

See full target information Goat IgG

대체 명칭 보기

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Methods in molecular biology (Clifton, N.J.) 2804:127-138 PubMed38753145

2024

Microfluidic Cartridge for Bead-Based Affinity Assays.

Applications

Unspecified application

Species

Unspecified reactive species

Inês F Pinto,Veronique Chotteau,Aman Russom

ACS sensors 6:842-851 PubMed33724791

2021

Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production.

Applications

Unspecified application

Species

Unspecified reactive species

Inês F Pinto,Ruben R G Soares,Meeri E-L Mäkinen,Veronique Chotteau,Aman Russom

European journal of pharmacology 762:82-8 PubMed26004535

2015

Non-uniform changes in membrane receptors in the rat urinary bladder following outlet obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwen Zeng,Mari Ekman,Chonghe Jiang,Bengt Uvelius,Karl Swärd

Dalton transactions (Cambridge, England : 2003) 44:4994-5003 PubMed25612290

2015

Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Stina Lindén,Manish Kumar Singh,K David Wegner,Marie Regairaz,François Dautry,François Treussart,Niko Hildebrandt
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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