Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750)
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(14 제품이 사용된 논문 )
Suitable for WB. Cited in 14 publications.
대체 명칭 보기
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1
- WB
Lab
Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) (AB175738)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175738 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes:
Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/ko/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL
All lanes:
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
All lanes:
Western blot - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750) (ab175738) at 1/10000 dilution
Predicted band size: 33 kDa,36 kDa,42 kDa,47 kDa,61 kDa,80 kDa
Observed band size: 100 kDa,50 kDa,60 kDa,74 kDa
false
Reactivity 정보
제품 세부 정보
We batch test Donkey Anti-Mouse IgG H&L (Alexa Fluor® 750), ab175738 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingDonkey Anti-Mouse IgG H&L (Alexa Fluor® 750), ab175738 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
특성 및 보관 정보
제형
Purification 테크닉
Purification 관련 사항
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
제품이 사용된 논문 (14)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Nature communications 16:8500 PubMed41006220
2025
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in neuroscience 18:1474617 PubMed39445075
2024
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Acta neuropathologica communications 12:132 PubMed39138580
2024
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International journal of molecular sciences 24: PubMed37686439
2023
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Cell reports 42:113029 PubMed37632750
2023
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Heliyon 9:e17907 PubMed37483799
2023
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Journal of chemical neuroanatomy 131:102284 PubMed37164181
2023
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Heliyon 9:e13315 PubMed36747525
2023
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Cancer research communications 2:172-181 PubMed36874403
2022
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Unspecified reactive species
Molecular oncology 16:2312-2329 PubMed34890102
2021
Applications
Unspecified application
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Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com