AB182020

Donkey Anti-Rabbit IgG H&L

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Suitable for WB, IP, IHC-P, ELISA.

대체 명칭 보기

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

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ELISA - Donkey Anti-Rabbit IgG H&L (AB182020)

ELISA

Lab

ELISA - Donkey Anti-Rabbit IgG H&L (AB182020)

Cross-reactivity of the polyclonal secondary antibody ab182020 was tested using a sandwich ELISA approach. The wells were coated with the indicated IgG standards at 1 μg/ml (50 μl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. ab182020 was then added starting at 1 μg/ml and gradually diluted 1/4 (50 μl/well), followed by incubation for 2h. For the detection Goat anti-Donkey IgG H&L (HRP) (ab6988) was used at 1/20,000 dilution (50 μl/well), followed by incubation for 1h at RT.

For the batch tested, ab182020 showed a cross-reactivity below 2% towards human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY.

주요 정보

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Applications

IHC-P, ELISA, WB, IP

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

The antibody reacts with rabbit immunoglobulins of all classes. Cross-reactions as determined by ELISA (see figure below): Human IgG, mouse IgG, rat IgG, goat IgG and chicken IgY, less than 2%.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/200000", "notes":"" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Immunogen affinity purified - This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Rabbit IgG

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rabbit IgG H&L

ELISA - Donkey Anti-Rabbit IgG H&L (AB182020)

주요 정보

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Applications

Clonality

Isotype

Specificity

대체품

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

보완 제품

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

Product promise