AB7082

Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed

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(4 제품이 사용된 논문 )

Donkey Anti-Rabbit IgG H&L (Biotin)(ab7082) is a biotin conjugated secondary antibody that is suitable for Western blot, IHC and ELISA.

- Preadsorbed to minimize non-specific binding and high background staining
- Suitable for avidin-biotin complex (ABC) method or labeled streptavidin-biotin (LSAB) method

1 이미지

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed (AB7082)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed (AB7082)

ab7082 was used at dilution 1/200 with the primary antibody ab77512 in IHC-Fr. See the review on ab77512.

주요 정보

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

ICC/IF, Dot, IM, IHC-Fr, WB, ELISA, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/20000 - 1/100000", "notes":"" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Rabbit IgG

대체 명칭 보기

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 7: PubMed33741591

2021

Persistent repression of tau in the brain using engineered zinc finger protein transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Susanne Wegmann,Sarah L DeVos,Bryan Zeitler,Kimberly Marlen,Rachel E Bennett,Marta Perez-Rando,Danny MacKenzie,Qi Yu,Caitlin Commins,Riley N Bannon,Bianca T Corjuc,Alison Chase,Lisa Diez,Hoang-Oanh B Nguyen,Sarah Hinkley,Lei Zhang,Alicia Goodwin,Annemarie Ledeboer,Stephen Lam,Irina Ankoudinova,Hung Tran,Nicholas Scarlott,Rainier Amora,Richard Surosky,Jeffrey C Miller,Ashley B Robbins,Edward J Rebar,Fyodor D Urnov,Michael C Holmes,Amy M Pooler,Brigit Riley,H Steve Zhang,Bradley T Hyman

Nanoscale advances 2:1253-1260 PubMed36133049

2020

Nanoparticle-based mobile biosensors for the rapid detection of sepsis biomarkers in whole blood.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Alba-Patiño,Steven M Russell,Marcio Borges,Nicolás Pazos-Pérez,Ramón A Álvarez-Puebla,Roberto de la Rica

Physiological reports 5: PubMed28400497

2017

C57BL/6J mice as a polygenic developmental model of diet-induced obesity.

Applications

ChIP

Species

Unspecified reactive species

Dinh-Toi Chu,Elzbieta Malinowska,Magdalena Jura,Leslie P Kozak

Cerebral cortex (New York, N.Y. : 1991) 25:3290-302 PubMed24927931

2014

Cortical and Clonal Contribution of Tbr2 Expressing Progenitors in the Developing Mouse Brain.

Applications

IHC (PFA fixed)

Species

Unspecified reactive species

Navneet A Vasistha,Fernando García-Moreno,Siddharth Arora,Amanda F P Cheung,Sebastian J Arnold,Elizabeth J Robertson,Zoltán Molnár

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (Biotin) preadsorbed (AB7082)

주요 정보

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

보완 제품

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise