Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

E14 mouse brain sample was stained with primary antibody (ab66155). The secondary antibody used was ab96919 (1 : 200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.

This image is courtesy of an anonymous Abreview

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

Glial fibrillary acidic protein(GFAP) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96919) was used as a secondary antibdoy at a dilution of 1/50.

This image is courtesy of an abreview submitted by Carl Hobbs, Kings's College London, United Kingdom.

• WB

Supplier Data

Western blot - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (AB96919)

All lanes:

rabbit anti-actin antibody at 1 µg/mL

All lanes:

HeLa whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96919) at 1/1000 dilution

false