AB6900

Donkey Anti-Sheep IgG H&L (HRP)

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(28 제품이 사용된 논문 )

Suitable for Dot, IM, ICC, WB, ELISA, IHC-P, IHC-Fr. Ideal for western blot. Cited in 28 publications.

2 이미지

ELISA - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ELISA

Supplier Data

ELISA - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ELISA results of purified ab6900 tested against purified Sheep IgG.

Each well was coated in duplicate with 1.0 μg of Sheep IgG. The starting dilution of antibody was 5 μg/ml and the X-axis represents the Log₁₀ of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC₅₀ is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

Unknown

Western blot - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.

Secondary

All lanes:

Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900) at 1/20000 dilution

false

주요 정보

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IHC-P, ELISA, WB, Dot, IHC-Fr, ICC, IM

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/3000 - 1/15000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/150000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

보관 버퍼

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Sheep IgG

대체 명칭 보기

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

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제품이 사용된 논문 (28)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eLife 13: PubMed40705007

2025

Coordinated transcriptional control of the adult ventricular conduction system.

Applications

Unspecified application

Species

Unspecified reactive species

Ozanna Burnicka-Turek,Katy A Trampel,Brigitte Laforest,Michael T Broman,Xinan H Yang,Zoheb Khan,Eric Rytkin,Binjie Li,Ella Schaffer,Margaret Gadek,Kaitlyn M Shen,Igor R Efimov,Ivan P Moskowitz

Cells 13: PubMed39451251

2024

AREG Upregulation in Cancer Cells via Direct Interaction with Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression Through EGFR-Erk/p38 MAPK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Nakanishi,Yu-Ichiro Koma,Shoji Miyako,Rikuya Torigoe,Hiroki Yokoo,Masaki Omori,Keitaro Yamanaka,Nobuaki Ishihara,Shuichi Tsukamoto,Takayuki Kodama,Mari Nishio,Manabu Shigeoka,Hiroshi Yokozaki,Yoshihiro Kakeji

Journal of tissue engineering and regenerative medicine 2024: PubMed38571695

2024

Genetically engineered macrophages derived from iPSCs for self-regulating delivery of anti-inflammatory biologic drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Molly Klimak,Farshid Guilak

The American journal of pathology 194:828-848 PubMed38320632

2024

Periostin in Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression by Enhancing Cancer and Stromal Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Shoji Miyako,Yu-Ichiro Koma,Takashi Nakanishi,Shuichi Tsukamoto,Keitaro Yamanaka,Nobuaki Ishihara,Yuki Azumi,Satoshi Urakami,Masaki Shimizu,Takayuki Kodama,Mari Nishio,Manabu Shigeoka,Yoshihiro Kakeji,Hiroshi Yokozaki

The Journal of biological chemistry 298:102676 PubMed36336076

2022

The stem region of α1,6-fucosyltransferase FUT8 is required for multimer formation but not catalytic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Seita Tomida,Masamichi Nagae,Yasuhiko Kizuka

Cell reports 40:111429 PubMed36170810

2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L Gibbons

Frontiers in endocrinology 13:960279 PubMed36034417

2022

Dexamethasone-associated metabolic effects in male mice are partially caused by depletion of endogenous corticosterone.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa L Koorneef,Merel van der Meulen,Sander Kooijman,Elena Sánchez-López,Jari F Scheerstra,Maaike C Voorhoeve,Ajith N Nadamuni Ramesh,Patrick C N Rensen,Martin Giera,Jan Kroon,Onno C Meijer

Hepatology communications 6:2689-2701 PubMed35833455

2022

Hepatic patatin-like phospholipase domain-containing 3 levels are increased in I148M risk allele carriers and correlate with NAFLD in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Elke Ericson,Linnéa Bergenholm,Anne-Christine Andréasson,Carly I Dix,Jane Knöchel,Sara F Hansson,Richard Lee,Jennifer Schumi,Madeleine Antonsson,Ola Fjellström,Patrik Nasr,Mathias Liljeblad,Björn Carlsson,Stergios Kechagias,Daniel Lindén,Mattias Ekstedt

Experimental and therapeutic medicine 23:277 PubMed35317443

2022

Monomeric C-reactive protein level is associated with osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Liang,Ke Xu,Wenguang Liu,Xiaoling Liu,Ping Yuan,Peng Xu,Haiyun Li

The Biochemical journal 478:3869-3889 PubMed34668531

2021

AMPK activation by SC4 inhibits noradrenaline-induced lipolysis and insulin-stimulated lipogenesis in white adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Ju Chuang,Manuel Johanns,Sébastien Pyr Dit Ruys,Gregory R Steinberg,Bruce E Kemp,Benoît Viollet,Mark H Rider

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Sheep IgG H&L (HRP)

ELISA - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

Western blot - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

주요 정보

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

보완 제품

Secondary Antibodies

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (28)

Product promise