Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab173004 Alexa Fluor® 647 goat anti-armenian hamster IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)

ICC/IF image of ab173004 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80952, 10μg/ml) overnight at +4°C. The secondary antibody (red) was ab173004 Alexa Fluor® 647 goat anti-armenian hamster IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)