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AB150162

Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

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Suitable for IHC-Fr, ICC/IF, Flow Cyt, IHC-P, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

대체 명칭 보기

Ig gamma-1 chain C region

2 이미지
Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150162, Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed, used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150162)

주요 정보

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ICC/IF, IHC-P, ELISA, IHC-Fr, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with the heavy and light chains of rat IgG, and with the light chains of rat IgM and IgA as demonstrated by ELISA, FLISA, and flow cytometry. Goat F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed shows minimal cross reactivity with mouse immunoglobulins.

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보완 제품

Primary Antibodies

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Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker

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Multiplex immunohistochemistry - Anti-LAMP1 antibody [EPR24395-31] - Lysosome Marker (AB278043)

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Primary Antibodies

AB192985

Anti-Histone H3 (tri methyl K27) antibody [EPR18607] - ChIP Grade

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ChIP-sequencing - Anti-Histone H3 (tri methyl K27) antibody [EPR18607] - ChIP Grade (AB192985)

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Primary Antibodies

AB2900

Anti-EEA1 antibody - Early Endosome Marker

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Western blot - Anti-EEA1 antibody - Early Endosome Marker (AB2900)

  • 4
  • 39
Primary Antibodies

AB137029

Anti-RAB7 antibody [EPR7589] - Late Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (AB137029)

  • 5
  • 25
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at an assay dependent dilution</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Use at an assay dependent dilution</p>" } } }
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제품 세부 정보

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Antiserum was solid phase adsorbed to ensure class specificity. Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
보관 버퍼
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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제품 프로토콜

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타겟 정보

See full target information Ig gamma-1 chain C region
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Current biology : CB 31:4713-4726.e4 PubMed34480856

2021

Evolution of crossover interference enables stable autopolyploidy by ensuring pairwise partner connections in Arabidopsis arenosa.

Applications

Unspecified application

Species

Unspecified reactive species

Chris Morgan,Martin A White,F Chris H Franklin,Denise Zickler,Nancy Kleckner,Kirsten Bomblies
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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