AB7152
Goat Anti-Human IgG H&L (Biotin) preadsorbed
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(6 제품이 사용된 논문 )
Suitable for WB, Dot, IHC-P, IM, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
1 이미지
- WB
Supplier Data
Western blot - Goat Anti-Human IgG H&L (Biotin) preadsorbed (AB7152)
Western blot analysis of ab7152. Secondary antibody : Peroxidase Streptavidin secondary antibody at 1/40000 dilution for 30 min at RT.
All lanes:
Western blot - Goat Anti-Human IgG H&L (Biotin) preadsorbed (ab7152) at 1/1000 dilution
All lanes:
Human IgG at 50 ng
Secondary
All lanes:
Peroxidase Streptavidin secondary antibody at 1/40000 dilution
false
보완 제품
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/2500", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200000", "notes":"<p></p>" } } }
특성 및 보관 정보
제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Secondary antibody conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
보관 버퍼
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
타겟 정보
Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.
Additional targets
IGHG3,IGHG2,IGHG4
대체 명칭 보기
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1, Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4, Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3, Immunoglobulin heavy constant gamma 2, Ig gamma-2 chain C region, Ig gamma-2 chain C region DOT, Ig gamma-2 chain C region TIL, Ig gamma-2 chain C region ZIE, IGHG2
제품이 사용된 논문 (6)
Recent publications for all applications. Explore the 전체 목록 and refine your search
ACS nano 17:23545-23567 PubMed37988765
2023
Single-Component Multilayered Self-Assembling Protein Nanoparticles Displaying Extracellular Domains of Matrix Protein 2 as a Pan-influenza A Vaccine.
Applications
Unspecified application
Species
Unspecified reactive species
Keegan Braz Gomes,Yi-Nan Zhang,Yi-Zong Lee,Mor Eldad,Alexander Lim,Garrett Ward,Sarah Auclair,Linling He,Jiang Zhu
Journal of pharmaceutical sciences 113:72-84 PubMed37844759
2023
Integrated PK/PD Modeling Relates Smoothened Inhibitor Biomarkers to The Heterogeneous Intratumor Disposition of Cetuximab in Pancreatic Cancer Tumor Models.
Applications
Unspecified application
Species
Unspecified reactive species
Jun Wang,Ting Chen,Donna M Ruszaj,Donald E Mager,Robert M Straubinger
Nature communications 14:1985 PubMed37031217
2023
Single-component multilayered self-assembling protein nanoparticles presenting glycan-trimmed uncleaved prefusion optimized envelope trimmers as HIV-1 vaccine candidates.
Applications
Unspecified application
Species
Unspecified reactive species
Yi-Nan Zhang,Jennifer Paynter,Aleksandar Antanasijevic,Joel D Allen,Mor Eldad,Yi-Zong Lee,Jeffrey Copps,Maddy L Newby,Linling He,Deborah Chavez,Pat Frost,Anna Goodroe,John Dutton,Robert Lanford,Christopher Chen,Ian A Wilson,Max Crispin,Andrew B Ward,Jiang Zhu
Science advances 7:eabj3107 PubMed34669468
2021
Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants.
Applications
Unspecified application
Species
Unspecified reactive species
Yi-Nan Zhang,Jennifer Paynter,Cindy Sou,Tatiana Fourfouris,Ying Wang,Ciril Abraham,Timothy Ngo,Yi Zhang,Linling He,Jiang Zhu
bioRxiv : the preprint server for biology : PubMed33791704
2021
Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants.
Applications
Unspecified application
Species
Unspecified reactive species
Yi-Nan Zhang,Jennifer Paynter,Cindy Sou,Tatiana Fourfouris,Ying Wang,Ciril Abraham,Timothy Ngo,Yi Zhang,Linling He,Jiang Zhu
Science translational medicine 5:187ra72 PubMed23720583
2013
Intranasal antibody gene transfer in mice and ferrets elicits broad protection against pandemic influenza.
Applications
Unspecified application
Species
Human
Maria P Limberis,Virginie S Adam,Gary Wong,Jason Gren,Darwyn Kobasa,Ted M Ross,Gary P Kobinger,Anna Tretiakova,James M Wilson
Product promise
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