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AB7148

Goat Anti-Human IgG H&L preadsorbed

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(3 제품이 사용된 논문 )

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
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ELISA - Goat Anti-Human IgG H&L preadsorbed (AB7148)
  • ELISA

Supplier Data

ELISA - Goat Anti-Human IgG H&L preadsorbed (AB7148)

ELISA results of ab7148 against purified Human IgG MX10. Each well was coated in duplicate with 1.0 µg of Human IgG [Red Line], Bovine IgG [Blue Line], Chicken IgG, Goat IgG [Purple Line], Guinea Pig IgG, Hamster IgG, Horse IgG, Mouse IgG, Rabbit IgG, Rat IgG, Sheep IgG. The working dilution is 1/184,500. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Donkey Anti-Goat IgG HRP conjugated and TMB substrate.

주요 정보

Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Hamster

Pre-adsorbed

Yes

Applications

Conjugation, IP, WB, ELISA, IHC-Fr, ID, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Specificity

<p>No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins.</p>

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보완 제품

Secondary Antibodies

AB98616

Goat Anti-Human IgG Fc preadsorbed

  • 0
  • 0
Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Secondary Antibodies

AB98624

Goat Anti-Human IgG Fc (HRP) preadsorbed

Western blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (AB98624)

  • 5
  • 2
Secondary Antibodies

AB97175

Goat Anti-Human IgG H&L (HRP) preadsorbed

  • 0
  • 0
Secondary Antibodies

AB7155

Rabbit Anti-Human IgG H&L preadsorbed

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/100000", "notes":"<p></p>" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
보관 버퍼
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

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타겟 정보

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.
See full target information IGHG1

Additional targets

IGHG2,IGHG3,IGHG4

대체 명칭 보기

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1, Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4, Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3, Immunoglobulin heavy constant gamma 2, Ig gamma-2 chain C region, Ig gamma-2 chain C region DOT, Ig gamma-2 chain C region TIL, Ig gamma-2 chain C region ZIE, IGHG2
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Methods in molecular biology (Clifton, N.J.) 2804:127-138 PubMed38753145

2024

Microfluidic Cartridge for Bead-Based Affinity Assays.

Applications

Unspecified application

Species

Unspecified reactive species

Inês F Pinto,Veronique Chotteau,Aman Russom

Turkish journal of biology = Turk biyoloji dergisi 47:130-140 PubMed37529164

2023

LINC00460 mediates HMGA2 expression through binding to miRNA-143-5p competitively in gastric carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xuqing Zhu,Yanli Xiang,Feifei Mo,Lingling Jin

ACS sensors 6:842-851 PubMed33724791

2021

Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production.

Applications

Unspecified application

Species

Unspecified reactive species

Inês F Pinto,Ruben R G Soares,Meeri E-L Mäkinen,Veronique Chotteau,Aman Russom
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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