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AB6785

Goat Anti-Mouse IgG H&L (FITC)

5

(1 리뷰)

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(642 제품이 사용된 논문 )

Goat anti-mouse IgG H&L (FITC) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.

- Cited in over 530 publications
6 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (AB6785)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (FITC) (AB6785)

ab6785 was used at dilution 1/300 with the primary antibody ab6002 in IHC-P. See the review on ab6002.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (AB6785)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (AB6785)

ab6785 was used at dilution 1/2000 with the primary antibody ab1220 in ICC/IF. See the review on ab1220.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (AB6785)
  • ICC/IF

AbReview29273****

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (FITC) (AB6785)

ab8978 staining vimentin in human colon fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol, permeabilized with 0.1% Triton X-100 and blocked with Dako serum free protein blocker for 20 minutes at 28°C. Samples were incubated with primary antibody (1/100 in Dako antibody diluent) for 2 hours at 28°C. A FITC-conjugated goat anti-mouse IgG H&L (ab6785) (1/800) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by J Chai.

Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (AB6785)
  • Flow Cyt

Unknown

Flow Cytometry - Goat Anti-Mouse IgG H&L (FITC) (AB6785)

ab6785 was used at dilution 1/100 with the primary antibody ab52455 in Flow Cyt. See the review on ab52455.

Western blot - Goat Anti-Mouse IgG H&L (FITC) (AB6785)
  • WB

Supplier Data

Western blot - Goat Anti-Mouse IgG H&L (FITC) (AB6785)

Western blot analysis of ab6785 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Mouse IgG at 50 ng

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785) at 1/1000 dilution

false

Dot Blot - Goat Anti-Mouse IgG H&L (FITC) (AB6785)
  • Dot

Supplier Data

Dot Blot - Goat Anti-Mouse IgG H&L (FITC) (AB6785)

Dot blot analysis of ab6785.

All lanes:

Dot Blot - Goat Anti-Mouse IgG H&L (FITC) (ab6785)

Lane 1:

Mouse IgG at 100 ng

Lane 2:

Mouse IgG at 33.3 ng

Lane 3:

Mouse IgG at 11.1 ng

Lane 4:

Mouse IgG at 3.7 ng

Lane 5:

Mouse IgG at 1.23 ng

Secondary

All lanes:

Goat Anti-Mouse IgG Fluorescein Conjugated Antibody at 1 µg/mL

false

주요 정보

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IM, IHC-Fr, WB, Dot, IHC-P, ELISA, IHC (PFA fixed), ICC/IF, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p>For PFA fixed tissue see PMID 15716365.</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"<p></p>" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC (PFA fixed)": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"<p>(PMID16006199)</p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/10000 - 1/50000", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Goat Anti-Mouse IgG [H&L] FITC Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
보관 버퍼
Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.424% Potassium phosphate solution, 0.01% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C

제품 프로토콜

타겟 정보

See full target information Ighg1

대체 명칭 보기

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

제품이 사용된 논문 (642)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 8:1446 PubMed41068254

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Cxcl9 macrophages recruit circulating Cxcr3+ plasmablasts into kidneys to promote pathogenesis of lupus nephritis mice.

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Jing Zhao,Xinlong Bai,Cheng Zhou,Qing Ouyang,Yingjie Zhang,Xiao Zhang,Xumin Zheng,Chaofan Li,Wanjun Shen,Qinggang Li,Guangyan Cai,Xiangmei Chen,Ping Li,Xue-Yuan Bai

Frontiers in molecular neuroscience 18:1603619 PubMed41030313

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Endoglin alleviates neuropathic pain by protecting the blood-spinal cord barrier through the TGF-β/Smad2 signaling pathway.

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SiJing Liao,Fei-Ran Zhou,Qun Li,Tao Jin,Gui-Fang Xiang,Xin Wang,Yan Liu,Hq Zhu,Qing Liu,Yuexin Liu,Ying Zhang

Stem cell research & therapy 16:507 PubMed41013769

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E2 pretreatment alleviates aggregation of intravenously injected mesenchymal stem cells in TBI by regulating BRG1 to affect adhesion.

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Wangan Li,Xiangyu Wang,Zhiming Feng,Shiting Hua,Xiaoxiong Zou,Zelin Lai,Yingqian Cai,Yuxi Zou,Yanping Tang,Cai Lin,Guobiao Luo,Xiaolin Cai,Xiaodan Jiang

Biology 14: PubMed40906142

2025

Effect of Recombinant NGF Encapsulated in Chitosan on Rabbit Sperm Traits and Main Metabolic Pathways.

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Species

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Luigia Bosa,Simona Mattioli,Anna Maria Stabile,Desirée Bartolini,Alessia Tognoloni,Alessandra Pistilli,Mariangela Ruggirello,Mario Rende,Silvia Gimeno-Martos,Daniela Jordán-Rodríguez,Maria Arias-Álvarez,Pilar García Rebollar,Rosa M García-García,Cesare Castellini

Journal of translational medicine 23:924 PubMed40826460

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AAV-mediated STC-1 expression mitigates neuroinflammation and preserves visual function in degenerative retinopathy.

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Yange Wang,Siyu Li,Chenxu Zhang,Miao Li,Kexin Wang,Gang Wang,Ye Tao,Zongming Song

Experimental & molecular medicine 57:1711-1726 PubMed40744997

2025

Extracellular vesicle-mediated delivery of circp53 suppresses the progression of multiple cancers by activating the CypD/TRAP/HSP90 pathway.

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Species

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Xichao Yu,Pinggang Ding,Mengjie Guo,Xiaozhu Tang,Ze Wang,Yuanjiao Zhang,Lianxin Zhou,Xinyu Lv,Hui Shi,Hongming Huang,Jialei Mao,Zhancheng Gu,Chunyan Gu,Ye Yang

Veterinary sciences 12: PubMed40711261

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Preparation of Monoclonal Antibodies Against the gD Protein of Feline Herpesvirus Type-1 by mRNA Immunization.

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Chengqi Zhang,Yawen Liu,Guangrong Zhao,Bo Hu,Liwen Xu,Jiajia Liu,Yajie Sun,Xiaolan Guo,Xiaoyu Deng,Shizhen Lian,Tiyun Han,Mengwei Xu,Shi Xu,Xue Bai

Frontiers in microbiology 16:1599691 PubMed40693141

2025

Tumor-associated bacteria activate PRDX1-driven glycolysis to promote immune evasion and PD-1 antibody resistance in hepatocellular carcinoma.

Applications

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Species

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Heng Zhang,Xi Lan,Liquan Cai,Xunfeng Gao,Feng Gao,Dan Yu,Jinlong Zhang,Jinhui Zhang,Qinwen Tai

Materials today. Bio 33:101995 PubMed40688680

2025

Inhibition of gastric adenocarcinoma proliferation by WSGC@MS: Role of KEAP1/NRF2 signaling pathway and autophagy regulation.

Applications

Unspecified application

Species

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Dongjian Song,Qiuliang Liu,Zechen Yan,Qi Wang,Meng Su,Hui Zhang,Longyan Shi,Yingzhong Fan,Qian Zhang,Heying Yang,Da Zhang

Journal of nanobiotechnology 23:519 PubMed40671009

2025

CXCL12-targeting siRNA nanoparticles alleviate immunosuppression and inhibit tumor progression in esophageal squamous cell carcinoma.

Applications

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Species

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Shuyao Zhang,Hong Jiang,Chengkuan Zhao,Yanli Lei,Shaojie Liu,Chengcheng Xu,Xiaoshan Chen,Danling Zheng,Xiaolong Wu,Xinyue Lin,Wang Chen,Yun Chen,Jianxiang Huang,XiaoLong Wei,Yihui Huang,Chaoxian Lin
제품이 사용된 논문 모두 보기

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