Goat Anti-Rabbit IgG (10nm Gold, 10 OD)
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(5 제품이 사용된 논문 )
Suitable for LFA. Cited in 5 publications.
대체 명칭 보기
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
- EM
PubMed
Electron Microscopy - Goat Anti-Rabbit IgG (10nm Gold, 10 OD) (AB270555)
Aparicio, I. M., et al. used Goat Anti-Rabbit IgG (10nm Gold, 10 OD) in electron transmission microscopy as part of examining whether autophagy-related proteins are present and functionally active in human spermatozoa.
Figures (a,b) are representative areas of the head. Figures (c,d,e) are digitally augmented images from mitochondria of the middle piece of the spermatozoa. Some of the LC3 proteins labeled with immunogold particles are marked with arrows; red arrows indicate gold particles (LC3) located on the membranes.
Image from Aparicio, I. M., et al., Sci Rep., 6:33647; doi: 10.1038/srep33647. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/
- LFA
Supplier Data
Lateral flow assay - Goat Anti-Rabbit IgG (10nm Gold, 10 OD) (AB270555)
Quantification of the T line signal of a lateral flow assay using ab270555.
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Reactivity 정보
제품 세부 정보
The Goat Anti-Rabbit IgG antibody (GAR) is covalently conjugated to 10 nm Gold Nanoparticles which have a unique protective coating that can withstand most extreme conditions (e.g. 2.5 M NaOH at 70°C for >1 hour). The irreversible attachment does not allow the desorption of the antibody, which is a major problem with products in which the antibody is only passively adsorbed to the metal.
To exchange the conjugated antibody into a different buffer specific to your application, centrifuge the conjugate in a microfuge at 21,500 x g for 45 minutes. Carefully remove the supernatant, gently tap the pellet and add the preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).
If an incubation of the conjugated Goat Anti-Rabbit IgG with the target antibody is performed, do not centrifuge the gold complex after this stage (an antibody titration is recommended).
The table below is just a guideline for possible applications:
| Size | LFA | FC | DLS | SEM | TEM |
| 80 nm | • | • | • | ||
| 40 nm | • | • | • | • | |
| 20 nm | • | • | • | • | |
| 10 nm | • | • | • |
This product is manufactured by Expedeon, an Abcam company. It was previously called 10nm InnovaCoat® GOLD (10 OD) Goat Anti Rabbit IgG Conjugate. Expedeon product code 218-0200 is the same as the 200 μl size of this product and code 218-1000 is the same as the 1 mL size.
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제품이 사용된 논문 (5)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Journal of biomedical science 32:69 PubMed40707961
2025
Applications
Unspecified application
Species
Unspecified reactive species
International journal of nanomedicine 20:1083-1100 PubMed39895982
2025
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Unspecified application
Species
Unspecified reactive species
Autophagy 20:2511-2528 PubMed39007910
2024
Applications
Unspecified application
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Unspecified reactive species
Autophagy 20:1723-1740 PubMed38566321
2024
Applications
Unspecified application
Species
Unspecified reactive species
Scientific reports 6:33647 PubMed27633131
2016
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com