Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed

• WB

Unknown

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

ab6940 was used at dilution 1/10000 with the primary antibody ab47840 in WB. See the review on ab47840.

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/10000 dilution

false

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Incubated with the primary antibody for 1 hour at room temperature.

Incubated with the secondary antibody for 30 minutes at room temperature.

Lane 1:

Rabbit IgG whole molecule at 0.05 µg

Lane 2:

Rabbit IgG F(ab) fragment at 0.05 µg

Lane 3:

Rabbit IgG F(c) fragment at 0.05 µg

Lane 4:

Rabbit IgM whole molecule at 0.05 µg

Lane 5:

Normal rabbit serum at 0.05 µg

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

Lanes 1 - 5:

Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution

false

• WB

Supplier Data

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (AB6940)

Incubated with ab6940 for 1 hour at room temperature.

All lanes:

Rabbit IgG at 0.05 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution

false