Goat Anti-Rabbit IgG H&L (HRP polymer)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma labelling Pan Trk with ab246551 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on human astrocytoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Oncostatin M/OSM with ab307951 at 1/100 (4.85 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human spleen. The section was incubated with ab307951 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Nectin 3 with ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human testis. The section was incubated with ab314219 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human diffuse large tissue labeling Oncostatin M/OSM with ab307951 at 1/100 (4.85 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human diffuse large B-cell lymphoma. The section was incubated with ab307951 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling Oncostatin M/OSM with ab307951 at 1/100 (4.85 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on scattered immune cells of human lung. The section was incubated with ab307951 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TRIP12/ULF with ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nuclear staining on human testis. The section was incubated with ab313628 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T c tissue labeling Oncostatin M/OSM with ab307951 at 1/100 (4.85 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on (A) HEK-293T transfected with a human Oncostatin M/OSM expression vector, no staining on (B) HEK-293T transfected with an empty vector. The section was incubated with ab307951 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Oncostatin M/OSM with ab307951 at 1/100 (4.85 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on scattered immune cells of human colon. The section was incubated with ab307951 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with ab256494 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on Human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle showing negative staining with purified ab131254 at 1/1800. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ab214880, a Goat Anti-Rabbit IgG H&L (HRP polymer) was used as the secondary antibody (1/500). Counterstained with hematoxylin. Negative control : No staining on human skeletal muscle. The section was incubated with ab131254 at 4°C overnight.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Nectin 3 with ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human lung carcinoma. The section was incubated with ab314219 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded (A) IMR-32 (human neuroblastoma neuroblast) cell pellet and (B) HeLa (human cervical adenocarcinoma epithelial cell) cell pellet labeling GPC2 with ab324378 at 1/200 (2.555 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining on (A) IMR-32 cell pellet, no staining on (B) HeLa cell pellet.
The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling Periostin with ab215199 at 1/1000 dilution (0.516 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at a Ready to use dilution. Positive staining on extracellular matrix of human breast carcinoma. The section was incubated with ab215199 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at Ready to use dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling AATF/DED with ab325343 at 1/100 (5.13 µg/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Nucleolus staining on human endometrial carcinoma. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Oncostatin M/OSM with ab307951 at 1/100 (4.85 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human breast. The section was incubated with ab307951 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling AATF/DED with ab325343 at 1/100 (5.13 µg/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Nucleolus staining on human colon carcinoma. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human pancreatic carcinoma tissue labelling CD133 with ab216323 at 1/1000 dilution (1.386 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at Ready to use dilution. Positive staining on human pancreatic carcinoma. The section was incubated with ab216323 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) at Ready to use dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma labelling Niemann Pick C2 with ab218192 at 1/8000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on Human breast carcinoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling PDGFR alpha with ab203491 A, B. at 1/100 dilution (5.81μg/ml) overnight at 4 °C, and C, at 1/2000 dilution (0.29 μg/ml) for 30 mins at room temperature. A, Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880) used as secondary (ready to use), B, C. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) used as secondary (ready to use). Counterstained using Hematoxylin. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Positive staining on human kidney. A, B : Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). C : Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

This antibody is better suited for Leica Biosystems BOND^® RX instrument. For manual detection, please use the Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on human tonsil The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Biotin with ab324266 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining in human pancreas tissue pre-incubated at 37°C for 1 hour with biotinylated Anti-Vimentin antibody [EPR3776] – Chicken IgY (Chimeric) (ab316214), followed by overnight incubation at 4°C with Rabbit monoclonal Anti-Biotin antibody [EPR28239-85] (ab324266) (Image A). In contrast, no positive staining is detected when the tissue is incubated only with the Anti-Biotin antibody (ab324266) under the same conditions (Image B).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil tissue labelling CD20 with ab78237 at a 1/100 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymoer) ab214880. The section was incubated with ab78237 overnight at 4°C.

The section was counterstained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Biotin with ab324266 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining in human breast tissue pre-incubated at 37°C for 1 hour with biotinylated Anti-Vimentin antibody [EPR3776] – Chicken IgY (Chimeric) (ab316214), followed by overnight incubation at 4°C with Rabbit monoclonal Anti-Biotin antibody [EPR28239-85] (ab324266) (Image A). In contrast, no positive staining is detected when the tissue is incubated only with the Anti-Biotin antibody (ab324266) under the same conditions (Image B).

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRPA1/TSA with ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human liver. The section was incubated with ab320715 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Digoxigenin with ab320717 at 1/1000 (0.503 ug/ml) dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining on (A) human tonsil incubated with mouse monoclonal PD-1 antibody conjugated to Digoxigenin and no staining on (B) human tonsil incubated with mouse monoclonal PD-1 antibody and (C) human tonsil incubated with PBS then all the sections were incubated with ab320717 at 4°C overnight.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling GPC2 with ab324378 at 1/200 (2.555 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Positive staining on human testis.
The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling TRPA1/TSA with ab320715 at 1/50 (9.98 ug/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human lung. The section was incubated with ab320715 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human liver. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human lung. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling AATF/DED with ab325343 at 1/100 (5.13 µg/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Nucleolus staining on human tonsil. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling AATF/DED with ab325343 at 1/100 (5.13 µg/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Nucleolus staining on human colon. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Nectin 3 with ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Negative control : no staining on human cardiac muscle. The section was incubated with ab314219 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TRIP12/ULF with ab313628 at 1/2000 (0.246 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Low expression : weakly nuclear staining on human liver. The section was incubated with ab313628 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human liver carcinoma tissue labeling AATF/DED with ab325343 at 1/100 (5.13 µg/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Nucleolus staining on human liver carcinoma. The primary antibody was incubated overnight at 4°C.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Oncostatin M/OSM with ab307951 at 1/100 (4.85 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on scattered immune cells of human liver. The section was incubated with ab307951 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP polymer) (AB214880)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Nectin 3 with ab314219 at 1/500 followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human placenta. The section was incubated with ab314219 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)