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AB175751

Goat Anti-Rat IgG H&L (Alexa Fluor® 750)

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(1 출판물)

Suitable for WB. Cited in 1 publication.

대체 명칭 보기

Ig gamma-1 chain C region

1 이미지
Western blot - Goat Anti-Rat IgG H&L (Alexa Fluor® 750) (AB175751)
  • WB

Lab

Western blot - Goat Anti-Rat IgG H&L (Alexa Fluor® 750) (AB175751)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab6160 overnight at 4°C. Antibody binding was detected using ab175751 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

false

주요 정보

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

WB

applications

Clonality

Polyclonal

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

제품 세부 정보

We batch test Goat Anti-Rat IgG H&L (Alexa Fluor® 750), ab175751 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Goat Anti-Rat IgG H&L (Alexa Fluor® 750), ab175751 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
보관 버퍼
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

제품 프로토콜

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

STAR protocols 6:103815 PubMed40381200

2025

Protocol for supervised automation of cell counting in confocal microscopic mice cochlear imaging datasets using macro in Imaris.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Taifur Rahman,Nashwaan Ali Khan,Ibrahim Razu,Mobin Ibne Mokbul,Shakila Mahmuda Fatima,Cristina L Garcia,Peter Eckard,Marlan R Hansen
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com