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AB214882

Goat Anti-Rat IgG H&L (HRP polymer)

5

(4 Reviews)

|

(10 Publications)

Goat Anti-Rat IgG H&L (HRP polymer) is a secondary antibody that is ideal for tissue imaging. Suitable for IHC and more.

- Reacts with heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins
- No cross-reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse

대체 명칭 보기

Ig gamma-1 chain C region

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CCXCR1/XCR1 with ab317578 at 1/100 (8.79 ug/ml) dilution, followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Positive staining on human Hodgkin's lymphoma.
The section was incubated with ab317578 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CCXCR1/XCR1 with ab317578 at 1/100 (8.79 ug/ml) dilution, followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Positive staining on human tonisl.
The section was incubated with ab317578 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CCXCR1/XCR1 with ab317578 at 1/100 (8.79 ug/ml) dilution, followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Negative control : No staining on human kidney.
The section was incubated with ab317578 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CCXCR1/XCR1 with ab317578 at 1/100 (8.79 ug/ml) dilution, followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Negative control : No staining on human skeletal muscle.
The section was incubated with ab317578 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded human corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in human corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD3 with ab11089 at 1/200 dilution, followed by a Goat Anti-Rat IgG H&L (HRP polymer) secondary (ab214882) ready to use.

Membranous staining on rat spleen.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3 with ab11089 at 1/200 dilution, followed by a Goat Anti-Rat IgG H&L (HRP polymer) secondary (ab214882) ready to use.

Membranous staining on mouse spleen.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemistry analysis of paraffin-embedded mouse lymph node tissue section labelling CSF-1-R with ab271294 at 1/200 dilution. The section was incubated with ab271294 at +4°C overnight. Goat Anti-Rat IgG H&L (HRP polymer) (ab214882) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Positive staining on the macrophages in mouse lymph node. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with anti dopamine receptor d1 antibody 11f11 se6 ihcp mouse cerebrumat 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rat IgG H&L (HRP polymer) (AB214882)

C57BL/6 mouse spleen stained with 1 μg/ml of rat anti-mouse CD45R/B220 detected using ab214882 Goat Anti-Rat IgG H&L (HRP polymer).

주요 정보

Host species

Goat

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

HRP polymer

Excitation/Emission
Applications

IHC-P, Ie

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

Full Length Protein corresponding to Rat Ig gamma-1 chain C region. The exact immunogen used to generate this antibody is proprietary information.

P20759

Specificity

<p>Based on IEP, ab214882 reacts with heavy (gamma) chains on rat IgG, light chains on all rat immunoglobulins. No cross-reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or mouse.</p>

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Ie": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This product is provided in a pre-diluted, ready-to-use format. Incubate 150 μl per slide for 1 hour.</p>" } } }

제품 세부 정보

This antibody reacts with the heavy and light chains of Rat IgG

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
ab214882 was affinity purified using solid phase Rat IgG. Purity >95% by SDS-PAGE.
보관 버퍼
Preservative: 0.1% Proclin 300 Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C

제품 프로토콜

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