AB99696

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)

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(2 제품이 사용된 논문 )

Suitable for WB, ELISA. Cited in 2 publications.

2 이미지

Unknown

Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (AB99696)

All lanes : Anti-beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) at 1 μg/ml

Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 μg per lane.

Secondary
Lanes 1 - 2 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/5000 dilution
Lanes 3 - 4 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/20000 dilution
Lanes 5 - 6 : Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG - light chain (AP) (ab99696) at 1/50000 dilution

Secondary

All lanes:

Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

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Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (AB99696)

Western blot showing that Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) specifically binds to light-chains

Left blot : Coomassie stain showing the rabbit heavy (~50kDa) and light-chains (~25 kDa)

Lane 1 : 10 μg rabbit monoclonal was loaded
Lane 2 : 5 μg rabbit monoclonal was loaded
Lane 3 : 2 μg rabbit monoclonal was loaded

Right blot : WB using Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (AP) (ab99696) as secondary antibody at 1/10,000

Lane 1 : 1 μg rabbit monoclonal was loaded
Lane 2 : 0.5 μg rabbit monoclonal was loaded
Lane 3 : 0.25 μg rabbit monoclonal was loaded

Secondary

All lanes:

Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (ab99696)

false

주요 정보

Host species

Mouse

Target species

Rabbit

Target isotype

IgG

Target specificity

Light chain

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

ELISA, WB

applications

Clonality

Monoclonal

Clone number

SB62a

Isotype

IgG2b

Light chain 타입

lambda

Specificity

ab99696 reacts with the light chain of native Rabbit IgG primary antibodies and does not bind to the reduced and denatured Rabbit IgG heavy chain band (50 kD).

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/4000", "notes":"<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 8 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.605% Tris, 0.0095% Magnesium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Rabbit IgG

대체 명칭 보기

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in microbiology 10:2161 PubMed31611848

2019

Arsenic Response of Three Altiplanic Strains With Different Tolerance Levels Against the Metalloid Species: A Proteomics Study.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Castro-Severyn,Coral Pardo-Esté,Yoelvis Sulbaran,Carolina Cabezas,Valentina Gariazzo,Alan Briones,Naiyulin Morales,Martial Séveno,Mathilde Decourcelle,Nicolas Salvetat,Francisco Remonsellez,Eduardo Castro-Nallar,Franck Molina,Laurence Molina,Claudia P Saavedra

Oncology reports 41:789-800 PubMed30535441

2018

lincRNA‑p21 inhibits the progression of non‑small cell lung cancer via targeting miR‑17‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Ao,Ming Jiang,Jie Zhou,Hongling Liang,Haoming Xia,Gang Chen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase)

Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (AB99696)

Western blot - Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (Alkaline Phosphatase) (AB99696)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Clone number

Isotype

Light chain 타입

Specificity

보완 제품

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise