Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) (AB169349)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. ab169349, Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) (red) was used at 1µg/ml for 1h at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) (AB169349)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal rabbit serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1μg/ml) overnight at +4°C. ab169349, Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) (red) was then used at 1μg/ml for 1h at room temperature. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

• Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) (AB169349)