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AB150143

Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647)

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(10 제품이 사용된 논문 )

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 10 publications.

대체 명칭 보기

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 이미지
Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150143)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150143)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5µg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150143 Alexa Fluor® 647 rabbit anti-goat IgG (H+L) (red) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150143)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Rabbit Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150143)

주요 정보

Host species

Rabbit

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

IHC-Fr, ELISA, IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }

제품 세부 정보

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
보관 버퍼
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

제품 프로토콜

제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:31922 PubMed40883493

2025

Car4-positive adipocyte progenitor cells adapt to the aging environment and work as protection against ROS via glutathione metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Masato Horino,Kenji Ikeda,Rei Okazaki,Yujiro Nakano,Ryo Kaneda,Ryoko Ishii,Jun Aoki,Kazunari Hara,Akira Takeuchi,Yoshihiro Niitsu,Kuniyuki Taguchi,Kuniyuki Kano,Masanori Murakami,Kazutaka Tsujimoto,Chikara Komiya,Tetsuya Yamada

Advanced healthcare materials 12:e2301799 PubMed37611966

2023

Exposure to Polystyrene Nanoplastics Led to Learning and Memory Deficits in Zebrafish by Inducing Oxidative Damage and Aggravating Brain Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Weishang Zhou,Difei Tong,Dandan Tian,Yingying Yu,Lin Huang,Weixia Zhang,Yihan Yu,Lingzheng Lu,Xunyi Zhang,Wangqi Pan,Jiawei Shen,Wei Shi,Guangxu Liu

Journal of cardiovascular development and disease 10: PubMed37367411

2023

Transgene Upregulates Expression to Promote Zebrafish Heart Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Zimu Tang,Kaiyuan Wang,Lijian Lo,Jun Chen

Cell death & disease 12:151 PubMed33542214

2021

Loss-of-function of p53 isoform Δ113p53 accelerates brain aging in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhao,Shengfan Ye,Zimu Tang,Liwei Guo,Zhipeng Ma,Yuxi Zhang,Chun Yang,Jinrong Peng,Jun Chen

Biomedicines 8: PubMed33352885

2020

3D-Hepatocyte Culture Applied to Parasitology: Immune Activation of Canine Hepatic Spheroids Exposed to .

Applications

Unspecified application

Species

Unspecified reactive species

Armanda V Rodrigues,Graça Alexandre-Pires,Ana Valério-Bolas,David Santos-Mateus,Mariana Rafael-Fernandes,Maria A Pereira,Dário Ligeiro,Telmo Nunes,Raquel Alves-Azevedo,Marcos Santos,Isabel Pereira da Fonseca,Gabriela Santos-Gomes

Cell death & disease 11:568 PubMed32703938

2020

p53 isoform Δ113p53 promotes zebrafish heart regeneration by maintaining redox homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shengfan Ye,Ting Zhao,Wei Zhang,Zimu Tang,Ce Gao,Zhipeng Ma,Jing-Wei Xiong,Jinrong Peng,Wei-Qiang Tan,Jun Chen

Biotechnology and applied biochemistry 68:92-101 PubMed32028539

2020

Combination of 5-azaytidine and hanging drop culture convert fat cell into cardiac cell.

Applications

Unspecified application

Species

Unspecified reactive species

Zahra Bagheri-Hosseinabadi,Fatemeh Seyedi,Hamid Reza Mollaei,Mojgan Moshrefi,Alexander Seifalian

Frontiers in immunology 10:950 PubMed31130950

2019

Reduced Angiopoietin-Like 4 Expression in Multiple Sclerosis Lesions Facilitates Lipid Uptake by Phagocytes via Modulation of Lipoprotein-Lipase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Alwin Kamermans,Merel Rijnsburger,Ananya Chakraborty,Susanne van der Pol,Helga E de Vries,Jack van Horssen

Nature materials 18:883-891 PubMed30886401

2019

Local nascent protein deposition and remodelling guide mesenchymal stromal cell mechanosensing and fate in three-dimensional hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Loebel,Robert L Mauck,Jason A Burdick

Cancer cell 32:761-776.e6 PubMed29232554

2017

Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor Abemaciclib.

Applications

Unspecified application

Species

Unspecified reactive species

Xueqian Gong,Lacey M Litchfield,Yue Webster,Li-Chun Chio,Swee Seong Wong,Trent R Stewart,Michele Dowless,Jack Dempsey,Yi Zeng,Raquel Torres,Karsten Boehnke,Cecilia Mur,Carlos Marugán,Carmen Baquero,Chunping Yu,Steven M Bray,Isabella H Wulur,Chen Bi,Shaoyou Chu,Hui-Rong Qian,Philip W Iversen,Farhana F Merzoug,Xiang S Ye,Christoph Reinhard,Alfonso De Dios,Jian Du,Charles W Caldwell,María José Lallena,Richard P Beckmann,Sean G Buchanan
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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