Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin)

• WB

Supplier Data

Western blot - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (AB238618)

This antibody reacts to non-reduced IgG1κ (~150 kDa), and slightly reacts to reduced κ light chain (~25 kDa).

Lane 1:

Non-reduced(-) mouse IgG1κ

Lane 2:

Reduced(+) mouse IgG1κ

Lane 3:

Non-reduced(-) mouse IgG1λ

Lane 4:

Reduced(+) mouse IgG1λ

Secondary

All lanes:

Western blot - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (ab238618) at 0.2 µg/mL

false

• ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (AB238618)

A titer ELISA of mouse IgG1κ. The plate was coated with different amounts of mouse IgG1κ. A serial dilution of ab238618 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

• ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM103] Anti-Mouse kappa light chain (Biotin) (AB238618)

ELISA of mouse immunoglobulins shows ab238618 reacts to the kappa light chain of mouse immuno-globulins. No cross reactivity with the lamda lightchain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immuno-globulins. 200 ng/ml, 50 ng/ml, or 10 ng/ml of ab238618 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.