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AB238532

Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin)

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Recombinant secondary antibody suitable for ELISA, WB.

대체 명칭 보기

IGHG2B, Ig gamma-2B chain C region, Igh-3, Immunoglobulin G2b, gamma2b, immunoglobulin heavy constant gamma 2B

3 이미지
ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)

A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)
  • ELISA

Supplier Data

ELISA - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)

ELISA of mouse immunoglobulins shows ab238532 reacts to the Fc region of mouse IgG2b; no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab238532 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Western blot - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)
  • WB

Supplier Data

Western blot - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (AB238532)

Lane 1:

Non-reduced mouse IgG2b at 20 µg

Lane 2:

Reduced mouse IgG2b at 20 µg

Secondary

All lanes:

Western blot - Rabbit monoclonal [RM108] Anti-Mouse IgG2b Fc (Biotin) (ab238532) at 0.2 µg/mL

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주요 정보

Host species

Rabbit

Target species

Mouse

Target isotype

IgG2b

Target specificity

Fc region

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

ELISA, WB

applications

Clonality

Monoclonal

Clone number

RM108

Isotype

IgG

Specificity

ab238532 reacts to the Fc region of mouse IgG2b. No cross reactivity with mouse IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. <br>The Fc region of RM108 has been engineered to eliminate Fc receptor binding.

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보완 제품

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Secondary Antibodies

AB272181

Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin)

Recombinant

ELISA - Goat monoclonal [RMG06] Anti-Mouse IgG Fc (Biotin) (AB272181)

  • 0
  • 0
Secondary Antibodies

AB238623

Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin)

ELISA - Rabbit monoclonal [RM106] Anti-Mouse IgG1 Fc (Biotin) (AB238623)

  • 0
  • 0
Primary Antibodies

AB190482

Anti-Mouse IgG2b antibody [RM108]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mouse IgG2b antibody [RM108] (AB190482)

  • 0
  • 0
Primary Antibodies

AB197780

Anti-mouse IgG Fc antibody [RMG06]

Recombinant

ELISA - Anti-mouse IgG Fc antibody [RMG06] (AB197780)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.005-0.2 µg/mL", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"0.1-0.5 µg/mL", "notes":"<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
ab238532 was purified from an animal origin–free culture supernatant.
보관 버퍼
Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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제품 프로토콜

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타겟 정보

See full target information Mouse IgG2b
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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