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AB6727

Rabbit Anti-Mouse IgG H&L (Biotin)

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(4 제품이 사용된 논문 )

Rabbit Anti-Mouse IgG H&L (Biotin) (ab6727) is a biotin conjugated secondary antibody that is suitable for ELISA applications.
1 이미지
ELISA - Rabbit Anti-Mouse IgG H&L (Biotin) (AB6727)
  • ELISA

Supplier Data

ELISA - Rabbit Anti-Mouse IgG H&L (Biotin) (AB6727)

ELISA results of ab6727 against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (green line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin HRP conjugate 1/10,000 and TMB-8000 substrate.

주요 정보

Host species

Rabbit

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

ELISA

applications

Clonality

Polyclonal

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보완 제품

Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
  • 5
Secondary Antibodies

AB207995

Goat Anti-Rabbit IgG H&L (Biotin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (AB207995)

  • 0
  • 0
Secondary Antibodies

AB6729

Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase)

  • 0
  • 0
Secondary Antibodies

AB6740

Rabbit Anti-Goat IgG H&L (Biotin)

ELISA - Rabbit Anti-Goat IgG H&L (Biotin) (AB6740)

  • 0
  • 0
Primary Antibodies

AB20920

Anti-Staphylococcus aureus antibody

Immunocytochemistry/ Immunofluorescence - Anti-Staphylococcus aureus antibody (AB20920)

  • 4
  • 8
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/300000", "notes":"<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
보관 버퍼
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

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타겟 정보

See full target information Ighg1

대체 명칭 보기

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1
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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JOR spine 8:e70054 PubMed40061888

2025

Application and Validation of Semiautomatic Quantification of Immunohistochemically Stained Sections for Low Cellular Tissue Such as Intervertebral Disc Using QuPath.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Nüesch,Maria Paola Ferri,Christine L Le Maitre

Cells 13: PubMed39272974

2024

The Expression of Toll-like Receptors in Cartilage Endplate Cells: A Role of Toll-like Receptor 2 in Pro-Inflammatory and Pro-Catabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Mengis,Laura Bernhard,Andrea Nüesch,Irina Heggli,Nick Herger,Jan Devan,Roy Marcus,Christoph J Laux,Florian Brunner,Mazda Farshad,Oliver Distler,Christine L Le Maitre,Stefan Dudli

JOR spine 3:e1098 PubMed33015573

2020

Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie Binch,Joseph Snuggs,Christine L Le Maitre

International journal of oncology 54:139-151 PubMed30431105

2018

HIF-1α contributes to tube malformation of human lymphatic endothelial cells by upregulating VEGFR-3.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Han,Jun Yan,Haini Chen,Yi Ji,Jianbing Chen,Jie Cui,Weimin Shen,Jijun Zou
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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