AB6746

Rabbit Anti-Sheep IgG H&L (Biotin)

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(7 제품이 사용된 논문 )

Rabbit Anti-Sheep IgG H&L (Biotin)(ab6746) is a biotin conjugated secondary antibody that is suitable for Western blot, IHC and ELISA.

- Suitable for avidin-biotin complex (ABC) method or labeled streptavidin-biotin (LSAB) method

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (Biotin) (AB6746)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (Biotin) (AB6746)

ab6746 was used at dilution 1/500 with the primary antibody ab9013 in IHC-P. See the review on ab9013.

주요 정보

Host species

Rabbit

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

IM, IHC-P, ICC/IF, Dot, WB, IHC-Fr, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/3000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/140000", "notes":"" } } }

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제품 세부 정보

Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Sheep IgG

대체 명칭 보기

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 40:111429 PubMed36170810

2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L Gibbons

Proceedings of the National Academy of Sciences of 117:6792-6800 PubMed32152097

2020

Intestinal bile acids directly modulate the structure and function of TcdB toxin.

Applications

Unspecified application

Species

Unspecified reactive species

John Tam,Simoun Icho,Evelyn Utama,Kathleen E Orrell,Rodolfo F Gómez-Biagi,Casey M Theriot,Heather K Kroh,Stacey A Rutherford,D Borden Lacy,Roman A Melnyk

Frontiers in microbiology 10:2790 PubMed31849920

2019

Omeprazole Increases the Efficacy of Acyclovir Against Herpes Simplex Virus Type 1 and 2.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Michaelis,Malte C Kleinschmidt,Denisa Bojkova,Holger F Rabenau,Mark N Wass,Jindrich Cinatl

Technology in cancer research & treatment 18:1533033819864311 PubMed31405336

2019

Inhibition of CD133 Overcomes Cisplatin Resistance Through Inhibiting PI3K/AKT/mTOR Signaling Pathway and Autophagy in CD133-Positive Gastric Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Lu,Gang Zhao,Yulong Yang,Zhaoyan Jiang,Jingli Cai,Hai Hu

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:14739-51 PubMed25355226

2014

BDNF-dependent plasticity induced by peripheral inflammation in the primary sensory and the cingulate cortex triggers cold allodynia and reveals a major role for endogenous BDNF as a tuner of the affective aspect of pain.

Applications

IHC

Species

Rat

Karine Thibault,Wee Khang Lin,Armelle Rancillac,Marie Fan,Thibaut Snollaerts,Vallier Sordoillet,Michel Hamon,George M Smith,Zsolt Lenkei,Sophie Pezet

Proceedings of the National Academy of Sciences of 110:12042-7 PubMed23818620

2013

Rotavirus mRNAS are released by transcript-specific channels in the double-layered viral capsid.

Applications

Unspecified application

Species

Unspecified reactive species

Javier Periz,Cristina Celma,Bo Jing,Justin N M Pinkney,Polly Roy,Achillefs N Kapanidis

The Journal of biological chemistry 280:5703-15 PubMed15590652

2004

p25alpha Stimulates alpha-synuclein aggregation and is co-localized with aggregated alpha-synuclein in alpha-synucleinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Evo Lindersson,Ditte Lundvig,Christine Petersen,Peder Madsen,Jens R Nyengaard,Peter Højrup,Torben Moos,Daniel Otzen,Wei-Ping Gai,Peter C Blumbergs,Poul Henning Jensen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rabbit Anti-Sheep IgG H&L (Biotin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Sheep IgG H&L (Biotin) (AB6746)

주요 정보

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

보완 제품

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise