Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)
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- Suitable for western blot (WB) and SDS-PAGE
- Suitable for Tris Glycine (4-12%) SDS buffer, Bis-Tris (4-12%) MOPS buffer and Bis-Tris (4-12%) MES buffer
- Part of our Lab essentials range western blot workflow
- SDS-PAGE
Supplier Data
SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)
SDS-PAGE analysis with ab234592.
Gel 1 : Tris-Glycine 4-20%
Gel 2 : Bis-Tris 4-12% MES buffer.
The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
보완 제품
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Reactivity 정보
제품 세부 정보
Prestained Protein Ladder ab234592 is a three-color protein standard with 10 pre-stained proteins covering a range of MW from 6.5-270 kDa. This prestained protein ladder allows you to monitor protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.
Key product features:
- Broad range: 6.5-270 kDa
- Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
- Easily identified: Proteins are covalently coupled with a blue chromophore except three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa).
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Review other protein ladders in the unstained and prestained protein ladder guide.
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제품이 사용된 논문 (1)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Scientific reports 10:14724 PubMed32895418
2020
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