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AB313995

Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa)

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Prestained Protein Ladder AB313995 is a combination of 9 pre-stained proteins with molecular weights from 15 to 170 kDa. This prestained protein ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
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SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (15 - 170 kDa) (AB313995)

Guide for Molecular Weight Estimation (kDa).

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note : The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

주요 정보

Applications

WB, SDS-PAGE

applications

제형

Liquid

form

보관 버퍼

pH: 7 - 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 25% Urea, 3% Sodium lauryl sulfate, 2% Tris phosphate, 0.016% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein migration and sizing of proteins on SDS-PAGE. Recommended loading amounts: 3-5 µl per well for 15-well or 10-well mini-gel, respectively.</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>For monitoring protein transfer onto membranes and sizing of proteins on WB. Recommended loading amounts: 2.5 μl.</p>" } } }

제품 세부 정보

Prestained Protein Ladder ab313995 is a combination of 9 pre-stained proteins with molecular weights from 15 to 170 kDa. This prestained protein ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Key Product Features: Mid-range molecular weight (15-170 kDa).
Ready to use: The ladder is supplied in the gel loading buffer and is ready to use.
Easily identified: The 9 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 15 kDa, a red band at 70 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C

제품 프로토콜

타겟 정보

Product promise

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