ELISA application page

View all ELISA resources.

From sample preparation to detailed protocols for different types of ELISAs.

ELISA sample preparation

Read our tips for preparing samples before running an ELISA in this detailed protocol.

Required controls for ELISA

Positive or negative? Spike or endogenous? Get the best possible results with your ELISA by using the right control samples with our complete guide.

Protocol for Sandwich ELISA

Learn how to set up a sandwich ELISA, covering all steps from plate coating and blocking to incubations with primary and secondary antibodies.

Protocol for indirect and direct ELISA

Our protocol for traditional ELISA formats using indirect and direct detection.

Protocol for direct ELISA using fluorescent substrate

Procedure and tips for direct ELISA assay using a fluorescent-conjugated primary antibody.

Protocol for in-cell ELISA

In-Cell ELISA (also known as cell-based ELISA, in cell western or cytoblot) is an immunocytochemistry method used to quantify target protein or post-translational modifications of the target protein, in cultured cells.

Protocol for ELISpot

General ELISpot procedure outlining plate and sample preparation, cell incubation, enzymatic detection, and analysis.

Competitive ELISA protocol

Competitive ELISA procedure summarizing antigen–antibody competition, signal generation inversely proportional to analyte concentration, and standard curve–based quantification.