Sample preparation guides by application

Biochemical and cell-based assays

Deproteinization

Cell proliferation and cell cytotoxicity

Wound healing assay

ChIP and chromatin analysis

Mammalian tissue preparation for ChIP

Native ChIP

Cross-linking ChIP-seq

ChIC/CUT&RUN-seq

Browse all ChIP and chromatin analysis resources

ELISA/immunoassay

Sample preparation for ELISA

Indirect and direct ELISA protocol

Sandwich ELISA protocol

ELISpot sample preparation

Browse all ELISA/immunoassay resources

Flow cytometry

Flow cytometry protocol

Practical guide for intracellular cytokine staining

Human and mouse B and T cell isolation

FMO protocol for panel validation

Browse all flow cytometry resources

Immunocytochemistry and immunofluorescence

2D cell culture ICC protocol

Spheroid preparation and immunofluorescence protocol

Browse all ICC/IF resources

Immunohistochemistry

Sample preparation for IHC

Best practice for tissue handling and fixation in IHC

IHC-P protocol

IHC-Fr protocol

Mouse-on-mouse IHC protocol

IHC staining for whole-mount samples

In-situ hybridization

Browse all IHC resources

Immunoprecipitation and co-IP

Immunoprecipitation protocol

RNA immunoprecipitation (RIP) protocol

Western blot

Sample preparation for western blot

Lysate preparation

Membrane-bound and nuclear protein sample preparation

Mitochondrial purification

Nuclear extraction and fractionation

Protein dephosphorylation

Secreted protein sample preparation