Anti-KPC2 antibody (ab151317)
Key features and details
- Rabbit polyclonal to KPC2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KPC2 antibody
See all KPC2 primary antibodies -
Description
Rabbit polyclonal to KPC2 -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIHC-P HumanWB Human -
Immunogen
A recombinant protein fragment corresponding to a region within amino acids 69 and 348 of Human KPC2 (UniProt ID: Q9BSL1).
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Positive control
- A549; HepG2 and HCT116 cell lysates; HeLa cells; Human hepatoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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ICC/IF |
Human
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IHC-P |
Human
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 10422 Human
- Omim: 608129 Human
- SwissProt: Q9BSL1 Human
- Unigene: 9194 Human
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Alternative names
- 1110033G07Rik antibody
- AA407978 antibody
- E3 ubiquitin-protein ligase subunit KPC2 antibody
see all
Images
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Anti-KPC2 antibody (ab151317) at 1/1000 dilution + A549 cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPC2 antibody (ab151317)Immunohistochemical analysis of paraffin-embedded Human hepatoma labeling KPC2 using ab151317 at 1/500 dilution.
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Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KPC2 using ab151317 at 1/200 dilution. Right panel costained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151317 has been referenced in 1 publication.
- Zhuang K et al. Gastrin induces multidrug resistance via the degradation of p27Kip1 in the gastric carcinoma cell line SGC7901. Int J Oncol 50:2091-2100 (2017). PubMed: 28498440