Anti-KPC2 antibody (ab151317)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-KPC2 antibody
See all KPC2 primary antibodies -
Description
Rabbit polyclonal to KPC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
A recombinant protein fragment corresponding to a region within amino acids 69 and 348 of Human KPC2 (UniProt ID: Q9BSL1).
-
Positive control
- A549; HepG2 and HCT116 cell lysates; HeLa cells; Human hepatoma tissue
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab151317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
-
Relevance
KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain. -
Cellular localization
Cytoplasmic -
Database links
- Entrez Gene: 10422 Human
- Omim: 608129 Human
- SwissProt: Q9BSL1 Human
- Unigene: 9194 Human
-
Alternative names
- 1110033G07Rik antibody
- AA407978 antibody
- E3 ubiquitin-protein ligase subunit KPC2 antibody
see all
Images
-
Western blot - Anti-KPC2 antibody (ab151317)Anti-KPC2 antibody (ab151317) at 1/1000 dilution + A549 cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPC2 antibody (ab151317)Immunohistochemical analysis of paraffin-embedded Human hepatoma labeling KPC2 using ab151317 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-KPC2 antibody (ab151317)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KPC2 using ab151317 at 1/200 dilution. Right panel costained with Hoechst 33342.
Protocols
References
This product has been referenced in:
- Zhuang K et al. Gastrin induces multidrug resistance via the degradation of p27Kip1 in the gastric carcinoma cell line SGC7901. Int J Oncol 50:2091-2100 (2017). Read more (PubMed: 28498440) »
