Anti-KPC2 antibody (ab151317)

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Western blot - Anti-KPC2 antibody (ab151317)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPC2 antibody (ab151317)
  • Immunocytochemistry/ Immunofluorescence - Anti-KPC2 antibody (ab151317)

Key features and details

  • Rabbit polyclonal to KPC2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-KPC2 antibody
    See all KPC2 primary antibodies

  • Description

    Rabbit polyclonal to KPC2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    A recombinant protein fragment corresponding to a region within amino acids 69 and 348 of Human KPC2 (UniProt ID: Q9BSL1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • A549; HepG2 and HCT116 cell lysates; HeLa cells; Human hepatoma tissue

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/1000.

Target

  • Relevance

    KPC2 (Kip1 ubiquitylation-promoting complex 2) together with KPC1 forms the ubiquitin ligase KPC, which regulates degradation of the cyclin-dependent kinase inhibitor p27 at the G1 phase of the cell cycle. KPC2 contains a ubiquitin-like (UBL) domain, two ubiquitin-associated (UBA) domains, and a heat shock chaperonin-binding (STI1) domain.

  • Cellular localization

    Cytoplasmic

  • Database links

    • Alternative names

      • 1110033G07Rik antibody
      • AA407978 antibody
      • E3 ubiquitin-protein ligase subunit KPC2 antibody
      • GBDR1 antibody
      • Glialblastoma cell differentiation-related protein 1 antibody
      • Glialblastoma differentiation-related protein 1 antibody
      • Kip1 ubiquitination-promoting complex protein 2 antibody
      • kpc 2 antibody
      • Kpc2 antibody
      • OTTHUMP00000022563 antibody
      • Putative glialblastoma cell differentiation-related protein antibody
      • RP11-432J22.3 antibody
      • UBA domain containing 1 antibody
      • UBA domain-containing protein 1 antibody
      • UBAC 1 antibody
      • UBAC1 antibody
      • Ubadc1 antibody
      • Ubiquitin associated domain containing 1 antibody
      • Ubiquitin-associated domain-containing protein 1 antibody
      see all

    Images

    • Western blot - Anti-KPC2 antibody (ab151317)
      Western blot - Anti-KPC2 antibody (ab151317)
      Anti-KPC2 antibody (ab151317) at 1/1000 dilution + A549 cell lysate at 30 µg

      Predicted band size: 45 kDa



      10% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPC2 antibody (ab151317)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPC2 antibody (ab151317)
      Immunohistochemical analysis of paraffin-embedded Human hepatoma labeling KPC2 using ab151317 at 1/500 dilution.
    • Immunocytochemistry/ Immunofluorescence - Anti-KPC2 antibody (ab151317)
      Immunocytochemistry/ Immunofluorescence - Anti-KPC2 antibody (ab151317)
      Immunofluorescence analysis of methanol-fixed HeLa cells, labeling KPC2 using ab151317 at 1/200 dilution. Right panel costained with Hoechst 33342.

    References (1)

    Publishing research using ab151317? Please let us know so that we can cite the reference in this datasheet.

    ab151317 has been referenced in 1 publication.

    • Zhuang K  et al. Gastrin induces multidrug resistance via the degradation of p27Kip1 in the gastric carcinoma cell line SGC7901. Int J Oncol 50:2091-2100 (2017). PubMed: 28498440

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